Journal article
In vivo analysis of fluid transport in cystic fibrosis airway epithelia of bronchial xenografts
The American journal of physiology, Vol.270(5 Pt 1), pp.C1326-1335
05/1996
DOI: 10.1152/ajpcell.1996.270.5.c1326
PMID: 8967432
Abstract
An in vivo human bronchial xenograft model system was used to simultaneously analyze electrolyte and fluid transport defects in fully differentiated human cystic fibrosis (CF) and non-CF proximal airways. CF airways demonstrated three discernible defects when compared with non-CF, including 1) a lack of adenosine 3',5'-cylic monophosphate (cAMP)-inducible Cl- secretion, 2) a fourfold higher basal fluid absorption rate, and 3) an altered regulation of fluid absorption in response to amiloride-stimulated changes in Na+ transport. A unique finding in this study demonstrated that treatment of epithelia with amiloride led to a greater than threefold decrease in the rate of fluid absorption in CF tissues as contrasted to a greater than threefold increase in the rate of fluid absorption in non-CF tissues. The removal of apical Na+ from amiloride-treated non-CF xenografts was capable of ablating this amiloride-induced increase in fluid absorption. In light of the recent interactions demonstrated between CF transmembrane conductance regulator (CFTR) and the rat epithelial, amiloride-sensitive Na+ channel, these findings implicate additional complexities between the Na+ conductance pathways and fluid transport in normal and CF proximal airways. Such findings suggest that CFTR may also regulate amiloride-insensitive Na+ channels.
Details
- Title: Subtitle
- In vivo analysis of fluid transport in cystic fibrosis airway epithelia of bronchial xenografts
- Creators
- Yulong Zhang - University of PennsylvaniaJames Yankaskas - University of PennsylvaniaJames Wilson - University of PennsylvaniaJohn F Engelhardt - University of Pennsylvania
- Resource Type
- Journal article
- Publication Details
- The American journal of physiology, Vol.270(5 Pt 1), pp.C1326-1335
- DOI
- 10.1152/ajpcell.1996.270.5.c1326
- PMID
- 8967432
- NLM abbreviation
- Am J Physiol
- ISSN
- 0002-9513
- eISSN
- 2163-5773
- Grant note
- P50 DK-49136 / NIDDK NIH HHS R01 DK047967 / NIDDK NIH HHS P30 DK-47757 / NIDDK NIH HHS
- Language
- English
- Date published
- 05/1996
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984284333502771
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