Journal article
In vivo and in vitro radiotherapy increased dentin enzymatic activity
Journal of dentistry, Vol.100, pp.103429-103429
09/01/2020
DOI: 10.1016/j.jdent.2020.103429
PMID: 32673637
Abstract
Objectives: The present study investigated the effects of in vitro and in-vivo radiotherapy on endogenous enzy-matic activity in dentin using gelatin zymography and in-situ zymography.
Methods: Gelatin zymographic assays were performed on protein extracts obtained from dentin powder of sound non-irradiated (NRT), in vitro irradiated (VTRT) and in vivo irradiated (VIRT) human teeth. Their proteolytic activities were quantified using band densitometric evaluation. For in-situ zymography, dentin specimens from NRT, VIRT and VTRT were covered with fluorescein-conjugated gelatin and examined with confocal laser -scanning microscopy. Fluorescence intensity emitted by the hydrolyzed fluorescein-conjugated gelatin was quantified and statistically analyzed. In-situ zymography data were statistically analyzed using Kruskal-Wallis ANOVA and Dunn's multiple comparison procedures (alpha = 0.05).
Results: No difference between in vitro and in vivo radiotherapy treatment was found. Both VTRT and VIRT groups showed increase in MMP-9 expression when compared to NRT group. Significant increases (p < 0.05) in gelatinolytic activity (26 % for VTRT; 55 % for VIRT) were observed when compared to the NRT group.
Conclusion: Radiotherapy increase endogenous enzymatic activity in non-restored dentin.
Details
- Title: Subtitle
- In vivo and in vitro radiotherapy increased dentin enzymatic activity
- Creators
- Sandra Ribeiro Cunha - University of IowaTatjana Maravic - University of BolognaAllegra Comba - University of BolognaPedro Augusto Ramos - Centro de Pesquisas OncológicasFranklin R. Tay - Augusta UniversityDavid H. Pashley - Augusta UniversityEduardo Rodrigues Fregnani - Hospital Sírio-LibanêsAna Cecilia Aranha - Universidade de São PauloAnnalisa Mazzoni - University of BolognaLorenzo Breschi - University of Bologna
- Resource Type
- Journal article
- Publication Details
- Journal of dentistry, Vol.100, pp.103429-103429
- Publisher
- Elsevier
- DOI
- 10.1016/j.jdent.2020.103429
- PMID
- 32673637
- ISSN
- 0300-5712
- eISSN
- 1879-176X
- Number of pages
- 6
- Grant note
- 2015/20297-3; 2017/01777-0 / FAPESP, Fundacao de Amparo a Pesquisa do Estado de Sao Paulo; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- Language
- English
- Date published
- 09/01/2020
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984696652902771
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