In vivo inhibition of nitric oxide synthase impairs upregulation of contractile protein mRNA in overloaded plantaris muscle

Jeff E Sellman; Keith C DeRuisseau; Jenna L Betters; Vitor A Lira; Quinlyn A Soltow; Joshua T Selsby; David S Criswell

doi:10.1152/japplphysiol.00936.2005

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In vivo inhibition of nitric oxide synthase impairs upregulation of contractile protein mRNA in overloaded plantaris muscle

Journal article

Peer reviewed

In vivo inhibition of nitric oxide synthase impairs upregulation of contractile protein mRNA in overloaded plantaris muscle

Jeff E Sellman, Keith C DeRuisseau, Jenna L Betters, Vitor A Lira, Quinlyn A Soltow, Joshua T Selsby and David S Criswell

Journal of applied physiology (1985), Vol.100(1), pp.258-265

01/2006

DOI: 10.1152/japplphysiol.00936.2005

PMID: 16166235

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Abstract

Inhibition of nitric oxide synthase (NOS) activity in vivo impedes hypertrophy in the overloaded rat plantaris. We investigated the mechanism for this effect by examining early events leading to muscle growth following 5 or 12 days of functional overload. Male Sprague-Dawley rats (approximately 350 g) were randomly divided into three treatment groups: control, N(G)-nitro-L-arginine methyl ester (L-NAME; 90 mg.kg(-1).day(-1)), and 1-(2-trifluoromethyl-phenyl)-imidazole (TRIM; 10 mg.kg(-1).day(-1)). Unilateral removal of synergists induced chronic overload (OL) of the right plantaris. Sham surgery performed on the left hindlimb served as a normally loaded control. L-NAME and TRIM treatments prevented OL-induced skeletal alpha-actin and type I (slow) myosin heavy chain mRNA expression at 5 days. Conversely, neither L-NAME nor TRIM affected hepatocyte growth factor or VEGF mRNA responses to OL at 5 days. However, OL induction of IGF-I and mechanogrowth factor mRNA was greater (P < 0.05) in the TRIM group compared with the controls. Furthermore, the phosphorylated-to-total p70 S6 kinase ratio was higher in OL muscle from NOS-inhibited groups, compared with control OL. At 12 days of OL, the cumulative proliferation of plantaris satellite cells was assessed by subcutaneous implantation of time release 5'-bromo-2'-deoxyuridine pellets during the OL-inducing surgeries. Although OL caused a fivefold increase in the number of mitotically active (5'-bromo-2'-deoxyuridine positive) sublaminar nuclei, this was unaffected by concurrent NOS inhibition. Therefore, NOS activity may provide negative feedback control of IGF-I/p70 S6 kinase signaling during muscle growth. Moreover, NOS activity may be involved in transcriptional regulation of skeletal alpha-actin and type I (slow) myosin heavy chain during functional overload.

Contractile Proteins - genetics

Muscular Atrophy

Nitric Oxide Synthase - antagonists & inhibitors

Rats

Male

Muscle, Skeletal - injuries

NG-Nitroarginine Methyl Ester - administration & dosage

RNA, Messenger - metabolism

Rats, Sprague-Dawley

Enzyme Inhibitors - administration & dosage

Animals

Muscle, Skeletal - drug effects

Muscle, Skeletal - physiopathology

Ankle Joint - physiopathology

Nitric Oxide Synthase - metabolism

Contractile Proteins - metabolism

Cumulative Trauma Disorders

Insulin-Like Growth Factor I - metabolism

Details

Title: Subtitle: In vivo inhibition of nitric oxide synthase impairs upregulation of contractile protein mRNA in overloaded plantaris muscle
Creators: Jeff E Sellman - P. O. Box 118206, Center for Exercise Science, University of Florida, Gainesville, Florida 32611, USA
Keith C DeRuisseau
Jenna L Betters
Vitor A Lira
Quinlyn A Soltow
Joshua T Selsby
David S Criswell
Resource Type: Journal article
Publication Details: Journal of applied physiology (1985), Vol.100(1), pp.258-265
DOI: 10.1152/japplphysiol.00936.2005
PMID: 16166235
NLM abbreviation: J Appl Physiol (1985)
ISSN: 8750-7587
eISSN: 1522-1601
Publisher: United States
Language: English
Date published: 01/2006
Academic Unit: Health, Sport, and Human Physiology
Record Identifier: 9984002338102771

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