Journal article
In vivo near-infrared spectroscopy of rat skin tissue with varying blood glucose levels
Analytical chemistry (Washington), Vol.78(1), pp.215-223
2006
DOI: 10.1021/ac051036i
PMID: 16383330
Abstract
We have performed in vivo measurements of near-infrared rat skin absorption in the 4000-5000-cm-1 spectral range (2.0-2.5-μm wavelength) during a glucose clamp experiment in order to identify the presence of glucose-specific spectral information. Spectra were collected during an initial 3-h period where the animal's blood glucose concentration was held at its normal value. The blood glucose level was then increased above 30 mM by venous infusion of glucose and held for 2 h, after which it was allowed to return to normal. Spectra were recorded continuously during the procedure and are analyzed to identify spectral changes associated with changes in glucose concentration. Because the change in absorbance due to an increase in glucose concentration is small compared to changes due to other variations (e.g., the thickness of the skin sample), a simple subtraction of absorbance spectra from the hyperglycemic and euglycemic phases is not instructive. Instead, a set of principal components is established from the euglycemic period where the glucose concentration is constant. We then examine the change in absorbance during the hyperglycemic period that is orthogonal to these principal components. We find that there are significant similarities between these orthogonal variations and the net analyte signal of glucose, which suggests that glucose spectral information is present. The analysis described here provides a procedure by which the analytical significance of a multivariate calibration can be evaluated. © 2006 American Chemical Society.
Details
- Title: Subtitle
- In vivo near-infrared spectroscopy of rat skin tissue with varying blood glucose levels
- Creators
- Jonathon T Olesberg - Optical Science and Technology Center and the Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, United StatesLINGZHI Liu - Optical Science and Technology Center and the Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, United StatesValerie VAN ZEE - Optical Science and Technology Center and the Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, United StatesMark A Arnold - Optical Science and Technology Center and the Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, United States
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.78(1), pp.215-223
- Publisher
- American Chemical Society
- DOI
- 10.1021/ac051036i
- PMID
- 16383330
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Language
- English
- Date published
- 2006
- Academic Unit
- Physics and Astronomy; Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216670202771
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