Journal article
In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem
The Journal of physiology, Vol.590(10), pp.2225-2231
05/2012
DOI: 10.1113/jphysiol.2011.226407
PMCID: PMC3424748
PMID: 22371480
Abstract
Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.
Details
- Title: Subtitle
- In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem
- Creators
- Daisuke Sugiyama - Shinshu UniversitySung Won Hur - Seoul National UniversityAnthony E. Pickering - University of BristolDaisuke Kase - National Institute for Physiological SciencesSang Jeong Kim - Seoul National UniversityMikito Kawamata - Shinshu UniversityKeiji Imoto - The Graduate University for Advanced Studies, SOKENDAIHidemasa Furue - The Graduate University for Advanced Studies, SOKENDAI
- Resource Type
- Journal article
- Publication Details
- The Journal of physiology, Vol.590(10), pp.2225-2231
- DOI
- 10.1113/jphysiol.2011.226407
- PMID
- 22371480
- PMCID
- PMC3424748
- NLM abbreviation
- J Physiol
- ISSN
- 0022-3751
- eISSN
- 1469-7793
- Publisher
- Wiley
- Number of pages
- 7
- Grant note
- National Research Foundation of Korea 2011-0030737 / Korea government; Korean Government 24390148; 21300127; 23390156; 23791693; 24390365 / Grants-in-Aid for Scientific Research; Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT); Japan Society for the Promotion of Science; Grants-in-Aid for Scientific Research (KAKENHI) Ministry of Education, Science, Sports and Culture of Japan; Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)
- Language
- English
- Date published
- 05/2012
- Academic Unit
- Anesthesia
- Record Identifier
- 9984787459802771
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