Journal article
In vivoDetection of Nitric Oxide and NOxSpecies Usingex vivoElectron Paramagnetic Resonance Spectroscopy
Microchemical journal, Vol.56(2), pp.165-170
06/1997
DOI: 10.1006/mchj.1997.1445
Abstract
Nitric oxide is a paramagnetic molecule that exhibits high affinity for heme proteins, forming a mixture of nitrosylated valency hybrids that can be precisely characterized using low-temperature electron paramagnetic resonance spectroscopy. In red blood cells, nitric oxide will form paramagnetic complexes with deoxyhemoglobin or be oxidized to nitrite and nitrate by oxyhemoglobin with formation of paramagnetic methemoglobin. We have developed a technique to monitor temporal changes in local nitric oxide productionin vivo,by sampling arterial and venous blood across a tissue bed and measuring changes in hemoglobin–nitric oxide and methemoglobin concentrationex vivousing electron paramagnetic resonance spectroscopy. Changes in local nitric oxide concentration are further clarified by quantifying plasma nitrite/nitrate levels. With these techniques, we have observed that whole body hyperthermia increases portal venous concentrations of hemoglobin–nitric oxide and organic radicals that are consistent with enhanced nitric oxide synthase activity, cytotoxic leukocyte activity, and cellular oxidative stress in splanchnic tissues.
Details
- Title: Subtitle
- In vivoDetection of Nitric Oxide and NOxSpecies Usingex vivoElectron Paramagnetic Resonance Spectroscopy
- Creators
- David M. Hall - Mallinckrodt Institute of Radiology, Section of Cancer Biology, Washington University School of Medicine, St. Louis, Missouri, 63108Garry R. Buettner - University of IowaCarl V. Gisolfi - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Microchemical journal, Vol.56(2), pp.165-170
- Publisher
- Elsevier B.V
- DOI
- 10.1006/mchj.1997.1445
- ISSN
- 0026-265X
- eISSN
- 1095-9149
- Language
- English
- Date published
- 06/1997
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984315644702771
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