Inactivation of Severe Acute Respiratory Coronavirus Virus 2 (SARS-CoV-2) and Diverse RNA and DNA Viruses on Three-Dimensionally Printed Surgical Mask Materials

Jennifer L Welch; Jinhua Xiang; Samantha R Mackin; Stanley Perlman; Peter Thorne; Patrick O'Shaughnessy; Brian Strzelecki; Patrick Aubin; Monica Ortiz-Hernandez; Jack T Stapleton

doi:10.1017/ice.2020.417

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Inactivation of Severe Acute Respiratory Coronavirus Virus 2 (SARS-CoV-2) and Diverse RNA and DNA Viruses on Three-Dimensionally Printed Surgical Mask Materials

Journal article

Open access

Peer reviewed

Inactivation of Severe Acute Respiratory Coronavirus Virus 2 (SARS-CoV-2) and Diverse RNA and DNA Viruses on Three-Dimensionally Printed Surgical Mask Materials

Jennifer L Welch, Jinhua Xiang, Samantha R Mackin, Stanley Perlman, Peter Thorne, Patrick O'Shaughnessy, Brian Strzelecki, Patrick Aubin, Monica Ortiz-Hernandez and Jack T Stapleton

Infection control and hospital epidemiology, Vol.42(3), pp.253-260

03/2021

DOI: 10.1017/ice.2020.417

PMCID: PMC7463154

PMID: 32783787

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Abstract

Personal protective equipment (PPE) is a critical need during the coronavirus disease 2019 (COVID-19) pandemic. Alternative sources of surgical masks, including 3-dimensionally (3D) printed approaches that may be reused, are urgently needed to prevent PPE shortages. Few data exist identifying decontamination strategies to inactivate viral pathogens and retain 3D-printing material integrity. To test viral disinfection methods on 3D-printing materials. The viricidal activity of common disinfectants (10% bleach, quaternary ammonium sanitizer, 3% hydrogen peroxide, or 70% isopropanol and exposure to heat (50°C, and 70°C) were tested on four 3D-printed materials used in the healthcare setting, including a surgical mask design developed by the Veterans' Health Administration. Inactivation was assessed for several clinically relevant RNA and DNA pathogenic viruses, including severe acute respiratory coronavirus virus 2 (SARS-CoV-2) and human immunodeficiency virus 1 (HIV-1). SARS-CoV-2 and all viruses tested were completely inactivated by a single application of bleach, ammonium quaternary compounds, or hydrogen peroxide. Similarly, exposure to dry heat (70°C) for 30 minutes completely inactivated all viruses tested. In contrast, 70% isopropanol reduced viral titers significantly less well following a single application. Inactivation did not interfere with material integrity of the 3D-printed materials. Several standard decontamination approaches effectively disinfected 3D-printed materials. These approaches were effective in the inactivation SARS-CoV-2, its surrogates, and other clinically relevant viral pathogens. The decontamination of 3D-printed surgical mask materials may be useful during crisis situations in which surgical mask supplies are limited.

DNA, Viral - drug effects

HIV-1 - drug effects

Humans

Hydrogen Peroxide

Hot Temperature

Healthy Volunteers

2-Propanol

Masks

COVID-19 - prevention & control

Disinfectants - pharmacology

Disinfection - methods

Personal Protective Equipment

SARS-CoV-2 - drug effects

Virus Inactivation

Decontamination - methods

Printing, Three-Dimensional

RNA, Viral - drug effects

Virus Diseases - prevention & control

Details

Title: Subtitle: Inactivation of Severe Acute Respiratory Coronavirus Virus 2 (SARS-CoV-2) and Diverse RNA and DNA Viruses on Three-Dimensionally Printed Surgical Mask Materials
Creators: Jennifer L Welch - Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Jinhua Xiang - Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Samantha R Mackin - Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Stanley Perlman - Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Peter Thorne - Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, Iowa
Patrick O'Shaughnessy - Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, Iowa
Brian Strzelecki - VA Puget Sound Health Care System, Seattle, Washington
Patrick Aubin - Department of Mechanical Engineering, University of Washington, Seattle, Washington
Monica Ortiz-Hernandez - Department of Mechanical Engineering, University of Washington, Seattle, Washington
Jack T Stapleton - Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Resource Type: Journal article
Publication Details: Infection control and hospital epidemiology, Vol.42(3), pp.253-260
DOI: 10.1017/ice.2020.417
PMID: 32783787
PMCID: PMC7463154
NLM abbreviation: Infect Control Hosp Epidemiol
ISSN: 0899-823X
eISSN: 1559-6834
Publisher: United States
Grant note: P30 ES005605 / NIEHS NIH HHS T32 AI007343 / NIAID NIH HHS R01 AI129269 / NIAID NIH HHS P01 AI060699 / NIAID NIH HHS
Language: English
Date published: 03/2021
Academic Unit: Civil and Environmental Engineering; Microbiology and Immunology; Occupational and Environmental Health; Infectious Diseases; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics); Internal Medicine
Record Identifier: 9984070256102771

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