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Incidence and Outcomes of Positive Donor Corneoscleral Rim Fungal Cultures after Keratoplasty
Journal article   Open access   Peer reviewed

Incidence and Outcomes of Positive Donor Corneoscleral Rim Fungal Cultures after Keratoplasty

Jesse M Vislisel, Kenneth M Goins, Michael D Wagoner, Gregory A Schmidt, Benjamin T Aldrich, Jessica M Skeie, Cynthia R Reed, M Bridget Zimmerman and Mark A Greiner
Ophthalmology (Rochester, Minn.), Vol.124(1), pp.36-42
01/2017
DOI: 10.1016/j.ophtha.2016.09.017
PMID: 27817919
url
https://doi.org/10.1016/j.ophtha.2016.09.017View
Published (Version of record) Open Access

Abstract

To determine the incidence of positive corneoscleral donor rim fungal cultures after keratoplasty and to report clinical outcomes of grafts with culture-positive donor rims. Retrospective cohort study. Consecutive donor corneas and keratoplasty recipients at a single tertiary referral center over 20 years. Patient charts were reviewed to determine the incidence of positive donor rim fungal cultures and clinical outcomes of all grafts using contaminated tissue. The primary outcome measures were positive donor rim fungal culture results and the development of postkeratoplasty fungal infection using corresponding corneal tissue. The secondary outcome measure was the impact of postoperative prophylaxis on donor tissue-associated infections. A total of 3414 keratoplasty cases were included in the statistical analysis. Seventy-one cases (2.1%) were associated with a fungal culture-positive donor rim. Candida species were cultured in 40 cases (56.3%). There was a higher incidence of positive rim cultures over the last 5 years of the analytic period compared with the first 15 years (P = 0.018). Fungal keratitis developed in 4 cases (5.6%), and all patients required further surgical intervention to achieve cure. There were no cases of fungal endophthalmitis. Empiric antimycotic prophylaxis initiated at the time of positive culture result reduced the incidence of keratitis from 15.8% in untreated cases to 1.9% in treated cases (P = 0.056). Positive donor rim fungal cultures are uncommon, but carry an unacceptably high risk of postoperative fungal infection. This risk may be reduced with prophylactic antimycotic therapy when culture-positive donor rims are identified.
Keratitis - microbiology Humans Middle Aged Fungi - isolation & purification Male Antibiotic Prophylaxis Keratitis - epidemiology Endophthalmitis - epidemiology Incidence Young Adult Anti-Bacterial Agents - therapeutic use Eye Infections, Fungal - prevention & control Aged, 80 and over Adult Eye Infections, Fungal - epidemiology Female Retrospective Studies Child Cornea - microbiology Keratoplasty, Penetrating - adverse effects Postoperative Complications - epidemiology Eye Infections, Fungal - microbiology Regression Analysis Postoperative Complications - microbiology Endophthalmitis - microbiology Adolescent Sclera - microbiology Aged Tissue Donors

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