Journal article
Incidence of anti-DNA idiotype-positive cells in human peripheral blood
Journal of clinical immunology, Vol.9(4), pp.313-321
07/1989
DOI: 10.1007/BF00918663
PMID: 2788658
Abstract
Human anti-DNA idiotype (Id)-bearing cells in peripheral blood were sought using mouse monoclonal anti-Id antibodies to human monoclonal anti-DNA antibodies. Pretreatment with acid pH or pronase P or preincubation in human serum-free medium markedly decreased the number of anti-Id-reactive cells. Pronase P-treated cells were able to bind to anti-Id once more after incubation for 18 hr at 37° C, indicating the resynthesis of internal idiotypic determinants on the cells. Antiidiotype-reactive cells retained the same idiotypes in their cytoplasma. The cells expressing anti-DNA idiotypes, termed 0-81 and NE-1, were detected in the circulation of most patients with active lupus nephritis but not in those of inactive systemic lupus erythematosus (SLE) and healthy subjects. The idiotype-positive cells occurred in up to 5–10% of B cells from some patients with SLE in the active stage but became undetectable in remission. A limited number of anti-DNA Id-positive cells responsible for anti-DNA production might be preferentially expanded during acute episodes of the disease in some patients with SLE.
Details
- Title: Subtitle
- Incidence of anti-DNA idiotype-positive cells in human peripheral blood
- Creators
- Osamu Takai - Tohoku UniversityTakeshi Sasaki - Tohoku UniversityTai Muryoi - Tohoku UniversityNaoko Harata - Tohoku UniversityHiroshi Hashimoto - Tohoku UniversityKaoru Yoshinaga - Tohoku University
- Resource Type
- Journal article
- Publication Details
- Journal of clinical immunology, Vol.9(4), pp.313-321
- DOI
- 10.1007/BF00918663
- PMID
- 2788658
- ISSN
- 0271-9142
- eISSN
- 1573-2592
- Language
- English
- Date published
- 07/1989
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984297508602771
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