Journal article
Incorporation of Adeno-Associated Virus in a Calcium Phosphate Coprecipitate Improves Gene Transfer to Airway Epithelia In Vitro and In Vivo
Journal of virology, Vol.74(1), pp.535-540
Note
01/2000
DOI: 10.1128/JVI.74.1.535-540.2000
PMCID: PMC111567
PMID: 10590145
Abstract
Adeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia because of a lack of receptors at the apical surface. We hypothesized that incorporation of AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly, coprecipitation of AAV type 2 improved gene transfer to differentiated human airway epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as a CaP
i
coprecipitate may significantly enhance its utility for gene transfer to the airway epithelia in vivo.
Details
- Title: Subtitle
- Incorporation of Adeno-Associated Virus in a Calcium Phosphate Coprecipitate Improves Gene Transfer to Airway Epithelia In Vitro and In Vivo
- Creators
- Robert W Walters - Howard Hughes Medical InstituteDongsheng Duan - Howard Hughes Medical InstituteJohn F Engelhardt - Howard Hughes Medical InstituteMichael J Welsh - Howard Hughes Medical Institute
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.74(1), pp.535-540
- Publisher
- American Society for Microbiology
- Series
- Note
- DOI
- 10.1128/JVI.74.1.535-540.2000
- PMID
- 10590145
- PMCID
- PMC111567
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 01/2000
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Anatomy and Cell Biology; Radiation Oncology; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020998802771
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