Incorporation of Non-Canonical Amino Acids

Lilia Leisle; Francis Valiyaveetil; Ryan A Mehl; Christopher A Ahern

doi:10.1007/978-1-4939-2845-3_7

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Incorporation of Non-Canonical Amino Acids

Journal article

Peer reviewed

Incorporation of Non-Canonical Amino Acids

Lilia Leisle, Francis Valiyaveetil, Ryan A Mehl and Christopher A Ahern

Advances in experimental medicine and biology, Vol.869, pp.119-151

2015

DOI: 10.1007/978-1-4939-2845-3_7

PMCID: PMC6005199

PMID: 26381943

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Abstract

In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical production of semisynthetic proteins have been used successfully for ion channel and receptor studies. The level of difficulty for the application of each approach ranges from trivial to technically demanding, yet all have untapped potential in their application to membrane proteins.

Amino Acid Sequence

Ion Channels - biosynthesis

Protein Engineering - methods

Humans

Amino Acids - chemistry

Ion Channels - genetics

Structure-Activity Relationship

Amino Acids - metabolism

Animals

Codon

Xenopus laevis - metabolism

Inteins

Xenopus laevis - genetics

Amino Acyl-tRNA Synthetases - metabolism

Ion Channels - chemistry

Details

Title: Subtitle: Incorporation of Non-Canonical Amino Acids
Creators: Lilia Leisle - Department of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Road, 52246, Iowa City, IA, USA
Francis Valiyaveetil - Department of Physiology and Pharmacology, Oregon Health and Sciences University, 97239, Portland, OR, USA
Ryan A Mehl - Department of Biochemistry and Biophysics, Oregon State University Corvallis, 97331, Corvallis, OR, USA
Christopher A Ahern - Department of Molecular Physiology and Biophysics, University of Iowa, 51 Newton Road, 52246, Iowa City, IA, USA. christopher-ahern@uiowa.edu
Resource Type: Journal article
Publication Details: Advances in experimental medicine and biology, Vol.869, pp.119-151
DOI: 10.1007/978-1-4939-2845-3_7
PMID: 26381943
PMCID: PMC6005199
NLM abbreviation: Adv Exp Med Biol
eISBN: 1493928457; 9781493928453
ISSN: 0065-2598
eISSN: 2214-8019
Publisher: United States
Grant note: R01 GM114653 / NIGMS NIH HHS R01 GM087546 / NIGMS NIH HHS R21 AR066802 / NIAMS NIH HHS R01 GM106569 / NIGMS NIH HHS
Language: English
Date published: 2015
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984065392602771

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