Journal article
Incorporation of copper into chitosan scaffolds promotes bone regeneration in rat calvarial defects
Journal of biomedical materials research. Part B, Applied biomaterials, Vol.103(5), pp.1044-1049
07/2015
DOI: 10.1002/jbm.b.33290
PMCID: PMC4363304
PMID: 25230382
Abstract
The objective of this study was to investigate the effects of a copper loaded chitosan scaffold on bone regeneration in critical-sized calvarial defects in rats. Chitosan scaffolds and copper-chitosan scaffolds were fabricated and characterized by scanning electron microscopy (SEM). Chitosan and copper-chitosan scaffolds were implanted into 5 mm diameter critical-sized calvarial defects in Fisher 344 male rats. Empty defects (no scaffolds) were included as a control. After 4 weeks, the rats were sacrificed for microcomputed tomography (micro-CT) and histological analysis of new bone tissue development. Microscopy images revealed the uniformly porous structure of chitosan and copper-chitosan scaffolds. Significant bone regeneration was noted in the defects treated with copper-chitosan scaffolds when evaluated using micro-CT and histological analysis, when compared with other groups tested. On analysis of the micro-CT scans, an eleven-fold and a two-fold increase in the new bone volume/total volume (BV/TV) % was found in defects treated with the copper-chitosan scaffolds, when compared to empty defects and chitosan scaffolds, respectively. This study demonstrated the suitability of copper-crosslinked chitosan scaffolds for bone tissue engineering and provides the first evidence that inclusion of copper ions in scaffolds can enhance tissue regeneration.
Details
- Title: Subtitle
- Incorporation of copper into chitosan scaffolds promotes bone regeneration in rat calvarial defects
- Creators
- Sheetal D'Mello - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa, 52242Satheesh Elangovan - Department of Periodontics, College of Dentistry, University of Iowa, Iowa City, Iowa, 52242Liu Hong - Department of Prosthodontics, College of Dentistry, University of Iowa, Iowa City, IowaRyan D Ross - Department of Anatomy and Cell Biology, Rush Medical College, Chicago, IllinoisD Rick Sumner - Department of Anatomy and Cell Biology, Rush Medical College, Chicago, IllinoisAliasger K Salem - Department of Periodontics, College of Dentistry, University of Iowa, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Journal of biomedical materials research. Part B, Applied biomaterials, Vol.103(5), pp.1044-1049
- DOI
- 10.1002/jbm.b.33290
- PMID
- 25230382
- PMCID
- PMC4363304
- NLM abbreviation
- J Biomed Mater Res B Appl Biomater
- ISSN
- 1552-4973
- eISSN
- 1552-4981
- Publisher
- United States
- Grant note
- 1R21CA13345-01/R21CA128414-01A2/UI / NCI NIH HHS\nR21 CA133456 / NCI NIH HHS\nR21 CA128414 / NCI NIH HHS
- Language
- English
- Date published
- 07/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Periodontics
- Record Identifier
- 9984065993402771
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