Journal article
Increased 8-hydroxydeoxyguanosine in hepatic DNA of rats treated with the peroxisome proliferators ciprofibrate and perfluorodecanoic acid
Cancer letters, Vol.87(2), pp.223-228
1994
DOI: 10.1016/0304-3835(94)90226-7
PMID: 7812944
Abstract
In this study we examined the ability of peroxisome proliferators to induce oxidative DNA damage in the form of 8-hydroxydeoxyguanosine (OHdG). We studied the hypolipidemic drug ciprofibrate, which is among the most potent and efficacious of the peroxisome proliferators, and perfluorodecanoic acid (PFDA), which is an inhibitor of peroxisomal β-oxidation. Rats were fed 0.01% ciprofibrate in the diet, or were injected with PFDA at doses of 3 or 10 mg/kg every 14 days (controls and ciprofibrate-fed rats were given equivalent doses of corn oil). Rats were maintained for 10 days, 24 days, 6 weeks, 26 weeks, or 54 weeks. DNA was isolated from the liver at these times and hydrolysed to nucleosides, and the levels of OHdG as well as normal nucleosides were analysed by high-performance liquid chromatography with electrochemical detection. Ciprofibrate increased OHdG concentrations at all times except for the initial 10-day timepoint. Both doses of PFDA increased OHdG levels at all times except the last timepoint, at which only the higher dose produced a significant increase. This study shows that both ciprofibrate and PFDA induce oxidative DNA damage in the form of OHdG. Furthermore, the inhibition of peroxisomal β-oxidation by PFDA does not affect the development of OHdG.
Details
- Title: Subtitle
- Increased 8-hydroxydeoxyguanosine in hepatic DNA of rats treated with the peroxisome proliferators ciprofibrate and perfluorodecanoic acid
- Creators
- Cheng-yu Huang - University of Kentucky, Department of Nutrition and Food Science, 219 Funkhouser Bldg., Lexington, KY 40506-0054, USAMary W Wilson - University of Kentucky, Department of Nutrition and Food Science, 219 Funkhouser Bldg., Lexington, KY 40506-0054, USAL.Travis Lay - University of Kentucky, Department of Nutrition and Food Science, 219 Funkhouser Bldg., Lexington, KY 40506-0054, USAChing K Chow - Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40506, USALarry W Robertson - Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40506, USAHoward P Glauert - Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40506, USA
- Resource Type
- Journal article
- Publication Details
- Cancer letters, Vol.87(2), pp.223-228
- Publisher
- Elsevier Ireland Ltd
- DOI
- 10.1016/0304-3835(94)90226-7
- PMID
- 7812944
- ISSN
- 0304-3835
- eISSN
- 1872-7980
- Language
- English
- Date published
- 1994
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9984002417802771
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