Journal article
Increased Concentration of Iodide in Airway Secretions Is Associated with Reduced Respiratory Syncytial Virus Disease Severity
American journal of respiratory cell and molecular biology, Vol.50(2), pp.389-397
02/2014
DOI: 10.1165/rcmb.2012-0529OC
PMCID: PMC3930944
PMID: 24053146
Abstract
Recent studies have revealed that the human and nonrodent mammalian airway mucosa contains an oxidative host defense system. This three-component system consists of the hydrogen peroxide (H
2
O
2
)-producing enzymes dual oxidase (Duox)1 and Duox2, thiocyanate (SCN
−
), and secreted lactoperoxidase (LPO). The LPO-catalyzed reaction between H
2
O
2
and SCN
−
yields the bactericidal hypothiocyanite (OSCN
−
) in airway surface liquid (ASL). Although SCN
−
is the physiological substrate of LPO, the Duox/LPO/halide system can generate hypoiodous acid when the iodide (I
−
) concentration is elevated in ASL. Because hypoiodous acid, but not OSCN
−
, inactivates respiratory syncytial virus (RSV) in cell culture, we used a lamb model of RSV to test whether potassium iodide (KI) could enhance this system
in vivo
. Newborn lambs received KI by intragastric gavage or were left untreated before intratracheal inoculation of RSV. KI treatment led to a 10-fold increase in ASL I
−
concentration, and this I
−
concentration was approximately 30-fold higher than that measured in the serum. Also, expiratory effort, gross lung lesions, and pulmonary expression of an RSV antigen and IL-8 were reduced in the KI-treated lambs as compared with nontreated control lambs. Inhibition of LPO activity significantly increased lesions, RSV mRNA, and antigen. Similar experiments in 3-week-old lambs demonstrated that KI administration was associated with reduced gross lesions, decreased RSV titers in bronchoalveolar lavage fluid, and reduced RSV antigen expression. Overall, these data indicate that high-dose KI supplementation can be used
in vivo
to lessen the severity of RSV infections, potentially through the augmentation of mucosal oxidative defenses.
Details
- Title: Subtitle
- Increased Concentration of Iodide in Airway Secretions Is Associated with Reduced Respiratory Syncytial Virus Disease Severity
- Creators
- Rachel J Derscheid - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IowaAlbert van Geelen - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IowaAbigail R Berkebile - Departments ofJack M Gallup - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IowaShannon J Hostetter - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IowaBotond Banfi - Anatomy and Cell Biology, andPaul B McCray - Departments ofMark R Ackermann - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.50(2), pp.389-397
- DOI
- 10.1165/rcmb.2012-0529OC
- PMID
- 24053146
- PMCID
- PMC3930944
- NLM abbreviation
- Am J Respir Cell Mol Biol
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Publisher
- American Thoracic Society
- Language
- English
- Date published
- 02/2014
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Otolaryngology; Internal Medicine
- Record Identifier
- 9984093355802771
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