Journal article
Increased Immunoreactive Rat Lung Icam-1 in Oleic Acid-Induced Lung Injury
Experimental lung research, Vol.21(4), pp.599-616
1995
DOI: 10.3109/01902149509031762
PMID: 7588446
Abstract
Levels of immunoreactive ICAM-1 in rat lung were followed during the kinetic development of acute oleic acid-induced lung injury in the rat by the ELISA assay. Significant increases in ICAM-1 immunoreactivity were found on rat lung membranes within 30 min of oleic acid injection. The increased immunoreactive ICAM-1 persisted for the duration of the study (4 h) and paralleled lung injury as measured by decreased lung compliance. Enhanced ICAM-1 immunofluorescence was also observed on cryostat sections of lungs from oleic acid-treated rats. No direct effect of oleic acid on ICAM-1 levels of cultured human umbilical vein endothelial cells or rat lung microvascular endothelial cells was observed. This suggests that either oleic acid raises rat lung ICAM-1 levels on endothelial cells by an indirect mechanism or that oleic acid increases ICAM-1 levels on other cell types, such as fibroblasts or lung epithelial cells, by direct or indirect mechanisms. Some of the increased ICAM-1 may also be due to the accumulation of ICAM-1 containing circulating leukocytes in the lung. The role of ICAM-1 in the pathophysiology of oleic acid-induced lung injury and the mechanism by which oleic acid increases ICAM-1 expression in the lung therefore remain to be defined by future experimentation.
Details
- Title: Subtitle
- Increased Immunoreactive Rat Lung Icam-1 in Oleic Acid-Induced Lung Injury
- Creators
- Sergei Syrbu - 1Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, USARoger S Thrall - 2Department of Surgery and Medicine, University of Connecticut Health Center, Farmington, Connecticut, USAPeter Wisniecki - 1Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, USAScott Lifchez - 1Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, USAHenry M Smilowitz - 1Department of Pharmacology, University of Connecticut Health Center, Farmington, Connecticut, USA
- Resource Type
- Journal article
- Publication Details
- Experimental lung research, Vol.21(4), pp.599-616
- Publisher
- Informa UK Ltd
- DOI
- 10.3109/01902149509031762
- PMID
- 7588446
- ISSN
- 0190-2148
- eISSN
- 1521-0499
- Language
- English
- Date published
- 1995
- Academic Unit
- Pathology
- Record Identifier
- 9984047730002771
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