Journal article
Increased Prostaglandin Synthesis by Low Calcium-Regulated Keratinocytes
Journal of investigative dermatology, Vol.86(2), pp.173-176
02/1986
DOI: 10.1111/1523-1747.ep12284218
PMID: 2427597
Abstract
The effect of calcium concentration on the in vitro prostaglandin production by murine keratinocytes was studied using radioimmunoassay. Keratinocytes grown in low-calcium medium (0.02 mM) maintained intracellular calcium levels adequate for arachidonic acid metabolism and actually showed increased prostaglandin production. Baseline, unstimulated PGE2 production was 4.5 times higher in cells growing in low- compared to normal-calcium (1.2 mM) medium (p = 0.001). PGF2α production was increased 2.5 times in the low-calcium cells (p = 0.002). The calcium ionophore A23187 and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) exhibited differing calcium requirements for activation of the arachidonic acid pathway. A23187 ionophore stimulated prostaglandin synthesis only in cells growing in normal-calcium medium while TPA stimulated prostaglandin production by both low- and normal-calcium cells. Paradoxically, short-term exposure of low calcium-grown cells to normal-calcium medium abolished the TPA effect. These results suggested that calcium can control arachidonic acid metabolism at a number of regulatory points.
Details
- Title: Subtitle
- Increased Prostaglandin Synthesis by Low Calcium-Regulated Keratinocytes
- Creators
- Janet A Fairley - University of RochesterJonathan Weiss - University of Michigan–Ann ArborCynthia L Marcelo - University of Michigan–Ann Arbor
- Resource Type
- Journal article
- Publication Details
- Journal of investigative dermatology, Vol.86(2), pp.173-176
- Publisher
- Elsevier Inc
- DOI
- 10.1111/1523-1747.ep12284218
- PMID
- 2427597
- ISSN
- 0022-202X
- eISSN
- 1523-1747
- Language
- English
- Date published
- 02/1986
- Academic Unit
- Dermatology
- Record Identifier
- 9984296309802771
Metrics
4 Record Views