Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping

Shawn W Flanagan; Pope L Moseley; Garry R Buettner

doi:10.1016/S0014-5793(98)00779-0

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Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping

Journal article

Open access

Peer reviewed

Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping

Shawn W Flanagan, Pope L Moseley and Garry R Buettner

FEBS letters, Vol.431(2), pp.285-286

1998

DOI: 10.1016/S0014-5793(98)00779-0

PMID: 9708920

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Abstract

It has been hypothesized that hyperthermia promotes oxygen-centered free radical formation in cells; however, to date there is no direct evidence of this heat-induced increase in oxygen free radical flux. Using electron paramagnetic resonance (EPR) spin trapping, we sought direct evidence for free radical generation during hyperthermia in intact, functioning cells. Rat intestinal epithelial cell monolayers were exposed to 45°C for 20 min, after which the nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) was added. Compared to control cells at 37°C, heat-exposed cells had increased free radical EPR signals, consistent with the formation of DMPO/ ⋅OH (a N=a H=14.9 G). These findings indicate that heat increases the flux of cellular free radicals and support the hypothesis that increased generation of oxygen-centered free radicals and the resultant oxidative stress may mediate in part, heat-induced cellular damage.

Heat injury

Oxidative stress

Electron paramagnetic resonance

Hyperthermia

Spin trapping

Free radical

Details

Title: Subtitle: Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping
Creators: Shawn W Flanagan - Departments of Exercise Science and Radiation Biology, 180B Medical Laboratories, The University of Iowa, Iowa City, IA 52242, USA
Pope L Moseley - Departments of Exercise Science and Radiation Biology, 180B Medical Laboratories, The University of Iowa, Iowa City, IA 52242, USA
Garry R Buettner - Departments of Exercise Science and Radiation Biology, 180B Medical Laboratories, The University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: FEBS letters, Vol.431(2), pp.285-286
DOI: 10.1016/S0014-5793(98)00779-0
PMID: 9708920
NLM abbreviation: FEBS Lett
ISSN: 0014-5793
eISSN: 1873-3468
Publisher: Elsevier B.V
Language: English
Date published: 1998
Academic Unit: Radiation Oncology; Health, Sport, and Human Physiology
Record Identifier: 9984046814702771

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