Journal article
Increased generation of lipid-derived and ascorbate free radicals by L1210 cells exposed to the ether lipid edelfosine
Cancer research (Chicago, Ill.), Vol.53(4), pp.711-713
1993
PMID: 8428351
Abstract
Using the spin trap alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone, we have detected a lipid-derived carbon-centered free radical generated from intact L1210 lymphoblastic leukemia cells that were exposed to 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine (edelfosine or ET-18-OCH3) and oxidative stress. The spectral characteristics, including hyperfine splitting constants of aN = 15.61G and aH = 2.65G, were consistent with the spin trapping of an alkyl radical. Radical detection required iron and prior enrichment of cellular components with the polyunsaturated fatty acid docosahexaenoic acid; unmodified cells failed to generate detectable free radical. Ascorbate further enhanced radical generation. The detection of lipid-derived free radicals when intact cells are exposed to edelfosine provides further evidence that oxidative stress may play an important role in the cytotoxic mechanism of this class of anticancer drug.
Details
- Title: Subtitle
- Increased generation of lipid-derived and ascorbate free radicals by L1210 cells exposed to the ether lipid edelfosine
- Creators
- B. A WAGNER - Univ. Iowa coll. medicine, dep. medicine, Iowa City IA 52242, United StatesG. R BUETTNER - Univ. Iowa coll. medicine, dep. medicine, Iowa City IA 52242, United StatesC. P BURNS - Univ. Iowa coll. medicine, dep. medicine, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.53(4), pp.711-713
- Publisher
- American Association for Cancer Research; Philadelphia, PA
- PMID
- 8428351
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 1993
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984046924002771
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