Journal article
Increased metabolic rate and insulin sensitivity in male mice lacking the carcino-embryonic antigen-related cell adhesion molecule 2
Diabetologia, Vol.55(3), pp.763-772
2012
DOI: 10.1007/s00125-011-2388-x
PMCID: PMC3272352
PMID: 22159884
Abstract
Aims/hypothesis
The carcino-embryonic antigen-related cell adhesion molecule (CEACAM)2 is produced in many feeding control centres in the brain, but not in peripheral insulin-targeted tissues. Global Ceacam2 null mutation causes insulin resistance and obesity resulting from hyperphagia and hypometabolism in female Ceacam2 homozygous null mutant mice (Cc2 [also known as Ceacam2]−/−) mice. Because male mice are not obese, the current study examined their metabolic phenotype.
Methods
The phenotype of male Cc2 −/− mice was characterised by body fat composition, indirect calorimetry, hyperinsulinaemic–euglycaemic clamp analysis and direct recording of sympathetic nerve activity.
Results
Despite hyperphagia, total fat mass was reduced, owing to the hypermetabolic state in male Cc2 −/− mice. In contrast to females, male mice also exhibited insulin sensitivity with elevated β-oxidation in skeletal muscle, which is likely to offset the effects of increased food intake. Males and females had increased brown adipogenesis. However, only males had increased activation of sympathetic tone regulation of adipose tissue and increased spontaneous activity. The mechanisms underlying sexual dimorphism in energy balance with the loss of Ceacam2 remain unknown.
Conclusions/interpretation
These studies identified a novel role for CEACAM2 in the regulation of metabolic rate and insulin sensitivity via effects on brown adipogenesis, sympathetic nervous outflow to brown adipose tissue, spontaneous activity and energy expenditure in skeletal muscle.
Details
- Title: Subtitle
- Increased metabolic rate and insulin sensitivity in male mice lacking the carcino-embryonic antigen-related cell adhesion molecule 2
- Creators
- P. R PATEL - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesS. K RAMAKRISHNAN - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesM. K KAW - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesC. K RAPHAEL - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesS GHOSH - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesJ. S MARINO - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesG HEINRICH - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesS. J LEE - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesR. E BOUREY - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesJ. W HILL - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United StatesD. Y JUNG - Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, United StatesD. A MORGAN - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA, United StatesJ K KIM - Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, United StatesS. K RAHMOUNI - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA, United StatesS. M NAJJAR - Center for Diabetes and Endocrine Research, College of Medicine and Life Sciences, University of Toledo, Health Science Campus, 3000 Arlington Avenue, Mail Stop 1009, Toledo, OH 43614, United States
- Resource Type
- Journal article
- Publication Details
- Diabetologia, Vol.55(3), pp.763-772
- Publisher
- Springer; Heidelberg
- DOI
- 10.1007/s00125-011-2388-x
- PMID
- 22159884
- PMCID
- PMC3272352
- ISSN
- 0012-186X
- eISSN
- 1432-0428
- Language
- English
- Date published
- 2012
- Academic Unit
- Neuroscience and Pharmacology
- Record Identifier
- 9984040479402771
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