Journal article
Increased mortality of acute respiratory distress syndrome was associated with high levels of plasma phenylalanine
Respiratory research, Vol.21(1), pp.99-99
04/30/2020
DOI: 10.1186/s12931-020-01364-6
PMCID: PMC7193408
PMID: 32354336
Abstract
There is a dearth of drug therapies available for the treatment of acute respiratory distress syndrome (ARDS). Certain metabolites play a key role in ARDS and could serve as potential targets for developing therapies against this respiratory disorder. The present study was designed to determine such "functional metabolites" in ARDS using metabolomics and in vivo experiments in a mouse model.
Metabolomic profiles of blood plasma from 42 ARDS patients and 28 healthy controls were captured using Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) assay. Univariate and multivariate statistical analysis were performed on metabolomic profiles from blood plasma of ARDS patients and healthy controls to screen for "functional metabolites", which were determined by variable importance in projection (VIP) scores and P value. Pathway analysis of all the metabolites was performed. The mouse model of ARDS was established to investigate the role of "functional metabolites" in the lung injury and mortality caused by the respiratory disorder.
The metabolomic profiles of patients with ARDS were significantly different from healthy controls, difference was also observed between metabolomic profiles of the non-survivors and the survivors among the ARDS patient pool. Levels of Phenylalanine, D-Phenylalanine and Phenylacetylglutamine were significantly increased in non-survivors compared to the survivors of ARDS. Phenylalanine metabolism was the most notably altered pathway between the non-survivors and survivors of ARDS patients. In vivo animal experiments demonstrated that high levels of Phenylalanine might be associated with the severer lung injury and increased mortality of ARDS.
Increased mortality of acute respiratory distress syndrome was associated with high levels of plasma Phenylalanine.
Chinese Clinical Trial Registry, ChiCTR1800015930. Registered 29 April 2018, http://www.chictr.org.cn/edit.aspx?pid=25609&htm=4.
Details
- Title: Subtitle
- Increased mortality of acute respiratory distress syndrome was associated with high levels of plasma phenylalanine
- Creators
- Jing Xu - Ruijin HospitalTingting Pan - Ruijin HospitalXiaoling Qi - Ruijin HospitalRuoming Tan - Ruijin HospitalXiaoli Wang - Shanghai Jiao Tong UniversityZhaojun Liu - Ruijin HospitalZheying Tao - Ruijin HospitalHongping Qu - Ruijin HospitalYi Zhang - China-Japan Friendship HospitalHong Chen - Ruijin HospitalYihui Wang - Ruijin HospitalJingjing Zhang - Ruijin HospitalJie Wang - Shanghai Jiao Tong UniversityJialin Liu - Ruijin Hospital
- Resource Type
- Journal article
- Publication Details
- Respiratory research, Vol.21(1), pp.99-99
- DOI
- 10.1186/s12931-020-01364-6
- PMID
- 32354336
- PMCID
- PMC7193408
- ISSN
- 1465-9921
- eISSN
- 1465-993X
- Grant note
- 18YF1413800 / Shanghai Sailing Program 8177010121 / National Natural Science Foundation of China 81772040 / National Natural Science Foundation of China 8180080170 / National Natural Science Foundation of China 8197010190 / National Natural Science Foundation of China 2016YFC1304303 / Key national R & D projects during the 13th five year plan
- Language
- English
- Date published
- 04/30/2020
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984696655002771
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