Increases in cytosolic Ca2+ in rat area postrema/mNTS neurons produced by angiotensin II and arginine-vasopressin

M Hay; G. L Edwards; K Lindsley; S Murphy; R. V Sharma; R. C Bhalla; A. K Johnson

doi:10.1016/0304-3940(93)90001-2

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Increases in cytosolic Ca2+ in rat area postrema/mNTS neurons produced by angiotensin II and arginine-vasopressin

Journal article

Peer reviewed

Increases in cytosolic Ca2+ in rat area postrema/mNTS neurons produced by angiotensin II and arginine-vasopressin

M Hay, G. L Edwards, K Lindsley, S Murphy, R. V Sharma, R. C Bhalla and A. K Johnson

Neuroscience letters, Vol.151(2), pp.121-125

1993

DOI: 10.1016/0304-3940(93)90001-2

PMID: 8506070

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Abstract

It is well established that neurons in the dorsal medulla, including the area postrema and the medial nucleus tractus solitarius (mNTS), are involved in the central actions of circulating peptides such as angiotensin II (ANG II) and arginine vasopressin (AVP). This report describes a reparation that permits the identification and maintenance of area postrema/mNTS neurons in culture in which the cellular and potentially subcellular responses to neurotransmitters and neuropeptides on area postrema/mNTS cells can be investigated. Following 15-21 days in culture, the effects of ANG II and AVP on changes in intracellular Ca2+ concentration ([Ca2+]i) were examined. Both ANG II and AVP resulted in a rapid and transient increase in [Ca2+]i reaching maximum in 15 s and returning towards baseline values within 180 s. The ANG II-mediated increase in [Ca2+]i was almost completely abolished by the selective angiotensin AT1 receptor subtype antagonist, losartan (DuP 753). These results suggest that ANG II and AVP modulate area postrema/mNTS neuronal activity by increasing intracellular Ca2+. © 1993.

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration

Vertebrates: nervous system and sense organs

Details

Title: Subtitle: Increases in cytosolic Ca2+ in rat area postrema/mNTS neurons produced by angiotensin II and arginine-vasopressin
Creators: M Hay - Univ. Iowa coll. medicine, cardiovascular cent., dep. internal medicine, Iowa City IA 52242, United States
G. L Edwards - Univ. Iowa coll. medicine, cardiovascular cent., dep. internal medicine, Iowa City IA 52242, United States
K Lindsley - Univ. Iowa coll. medicine, cardiovascular cent., dep. internal medicine, Iowa City IA 52242, United States
S Murphy - Univ. Iowa coll. medicine, cardiovascular cent., dep. internal medicine, Iowa City IA 52242, United States
R. V Sharma - Univ. Iowa coll. medicine, cardiovascular cent., dep. internal medicine, Iowa City IA 52242, United States
R. C Bhalla - Univ. Iowa coll. medicine, cardiovascular cent., dep. internal medicine, Iowa City IA 52242, United States
A. K Johnson - Univ. Iowa coll. medicine, cardiovascular cent., dep. internal medicine, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: Neuroscience letters, Vol.151(2), pp.121-125
Publisher: Elsevier
DOI: 10.1016/0304-3940(93)90001-2
PMID: 8506070
ISSN: 0304-3940
eISSN: 1872-7972
Language: English
Date published: 1993
Academic Unit: Anatomy and Cell Biology; Psychological and Brain Sciences; Neuroscience and Pharmacology; Health and Human Physiology
Record Identifier: 9984213425102771

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