Induced differentiation of a transformed clone of Ly-1+ B cells by clonal T cells and antigen

G A Bishop; G Haughton

doi:10.1073/pnas.83.19.7410

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Induced differentiation of a transformed clone of Ly-1+ B cells by clonal T cells and antigen

Journal article

Open access

Peer reviewed

Induced differentiation of a transformed clone of Ly-1+ B cells by clonal T cells and antigen

G A Bishop and G Haughton

Proceedings of the National Academy of Sciences - PNAS, Vol.83(19), pp.7410-7414

10/1986

DOI: 10.1073/pnas.83.19.7410

PMCID: PMC386727

PMID: 2945204

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https://europepmc.org/articles/pmc386727View

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Abstract

The present study used cocultures of clonally derived B and T cells, together with an antigen reactive with the membrane Ig of the clonal B cells, to address the issue of B-cell differentiation requirements. The B cells were CH12.LX, an in vitro grown subclone of a murine B-cell lymphoma, which bears surface IgM reactive with sheep erythrocytes. The T cells were alloreactive T-helper-cell hybridomas. Very small numbers of T-helper cells could induce differentiation of cloned B cells without the presence of accessory cells, but such induction was dependent upon the presence of the antigen recognized by the B cell. Induced differentiation of the B cells did not depend on metabolic activity of the T cells, and metabolically active T cells could be replaced by fixed cells or by monoclonal antibody reactive with the class II molecule of the B cell to deliver an Ia-specific differentiative signal. T cells, or alloantibody that reacted with the I-E molecule, induced differentiation of the B cells; those that reacted with the I-A molecule did not. These results define the minimal requirements for major histocompatibility complex-restricted, T-cell-mediated induction of B-cell differentiation.

Antigens

Cell Differentiation

Interleukin-2 - metabolism

B-Lymphocytes - cytology

Cells, Cultured

Antigens, Ly - analysis

Animals

B-Lymphocytes - immunology

Histocompatibility Antigens Class II - immunology

Fixatives

T-Lymphocytes, Helper-Inducer - immunology

Lymphocyte Cooperation

T-Lymphocytes - immunology

Mice

Details

Title: Subtitle: Induced differentiation of a transformed clone of Ly-1+ B cells by clonal T cells and antigen
Creators: G A Bishop
G Haughton
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.83(19), pp.7410-7414
DOI: 10.1073/pnas.83.19.7410
PMID: 2945204
PMCID: PMC386727
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; United States
Grant note: CA23770 / NCI NIH HHS CA29664 / NCI NIH HHS
Language: English
Date published: 10/1986
Academic Unit: Microbiology and Immunology; President
Record Identifier: 9984002384602771

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