Journal article
Inducible Expression of the gp49B Inhibitory Receptor on NK Cells
The Journal of immunology (1950), Vol.164(10), pp.5215-5220
05/15/2000
DOI: 10.4049/jimmunol.164.10.5215
PMID: 10799881
Abstract
Murine NK cells express inhibitory receptors belonging to the C-type lectin-like (Ly-49, CD94/NKG2) and Ig superfamily-related (gp49) receptors. The murine gp49B receptor displays structural homology with human killer inhibitory receptors, and was previously identified to be a receptor on mast cells and activated NK cells. The gp49B receptor is highly related to gp49A, a receptor with unknown function. In this study, using a novel mAb produced against soluble gp49B molecules that cross-reacts with gp49A, we examined the cellular distribution and function of these receptors. gp49 is constitutively expressed on cells of the myeloid lineage throughout development, as well as on mature cells. Importantly, gp49 is not expressed on spleen- and liver-derived lymphocytes, including NK cells, but its expression is induced in vitro on NK cells following IL-2 stimulation, or in vivo by infection with murine CMV. Molecular studies revealed that both the immunoreceptor tyrosine-based inhibitory motif-containing gp49B as well as immunoreceptor tyrosine-based inhibitory motif-less gp49A receptors are up-regulated on NK cells following murine CMV infection. When co-cross-linked with NK1.1, gp49B can inhibit NK1.1-mediated cytokine release by NK cells. Taken together, these studies demonstrate that the expression of gp49B on NK cells is regulated, providing the first example of an in vivo activation-induced NK cell inhibitory receptor, in contrast to the constitutively expressed Ly49 family.
Details
- Title: Subtitle
- Inducible Expression of the gp49B Inhibitory Receptor on NK Cells
- Creators
- Lawrence L. Wang - Howard Hughes Medical InstituteDortha T. Chu - Howard Hughes Medical InstituteAyotunde O. Dokun - Howard Hughes Medical InstituteWayne M. Yokoyama - Howard Hughes Medical Institute
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.164(10), pp.5215-5220
- DOI
- 10.4049/jimmunol.164.10.5215
- PMID
- 10799881
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 05/15/2000
- Academic Unit
- Molecular Physiology and Biophysics; Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984297603002771
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