Inducible mutagenesis in TEPC 2372, a mouse plasmacytoma cell line that harbors the transgenic shuttle vector λLIZ

K Felix; A.L Kovalchuk; S.S Park; A.E Coleman; E.S Ramsay; M Qian; K.A Kelliher; G.M Jones; T Ried; G.W Bornkamm; S Janz

doi:10.1016/S0027-5107(00)00143-3

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Journal article

Inducible mutagenesis in TEPC 2372, a mouse plasmacytoma cell line that harbors the transgenic shuttle vector λLIZ

K Felix, A.L Kovalchuk, S.S Park, A.E Coleman, E.S Ramsay, M Qian, K.A Kelliher, G.M Jones, T Ried, G.W Bornkamm, …

Mutation research, Vol.473(1), pp.121-136

2001

DOI: 10.1016/S0027-5107(00)00143-3

PMID: 11166031

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Abstract

The plasmacytoma cell line, TEPC 2372, was derived from a malignant plasma cell tumor that developed in the peritoneal cavity of a BALB/c mouse that harbored the transgenic shuttle vector for the assessment of mutagenesis in vivo, λLIZ. TEPC 2372 was found to display the typical features of a BALB/c plasmacytoma. It consisted of pleomorphic plasma cells that secreted a monoclonal immunoglobulin (IgG2b/λ), was initially dependent on the presence of IL-6 to grow in cell culture, contained a hyperdiploid chromosome complement with a tendency to undergo tetraploidization, and harbored a constitutively active c- myc gene by virtue of a T(6;15) chromosomal translocation. TEPC 2372 was further characterized by the ability to respond to in vitro exposure with 4-NQO (4-nitroquinoline-1-oxide), an oxidative model mutagen, with a vigorous dose-dependent increase in mutagenesis that peaked at a 7.85-fold elevation of mutant rates in λLIZ when compared to background mutant rates in untreated controls. Cotreatment with 4-NQO and BSO (buthionine sulfoximine), a glutathione-depleting compound that causes endogenous oxidative stress, resulted in a 9.03-fold increase in the mutant frequency in λLIZ. These results demonstrated that TEPC 2372, the malignant plasma cell counterpart of the λLIZ-based in vivo mutagenesis assay, may be useful as an in vitro reference point for the further elucidation of oxidative mutagenesis in lymphoid tissues.

BALB/c mice

λLIZ-based transgenic mutagenicity assay

Pristane (2,6,10,14-tetramethylpentadecane)

Peritoneal plasmacytomagenesis

Details

Title: Subtitle: Inducible mutagenesis in TEPC 2372, a mouse plasmacytoma cell line that harbors the transgenic shuttle vector λLIZ
Creators: K Felix - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
A.L Kovalchuk - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
S.S Park - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
A.E Coleman - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
E.S Ramsay - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
M Qian - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
K.A Kelliher - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
G.M Jones - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
T Ried - Medicine Branch, NCI, NIH, Bethesda, MD, USA
G.W Bornkamm - Institute of Molecular Biology and Tumor Genetics, GSF, Munich, Germany
S Janz - Laboratory of Genetics, DBS, NCI, Building 37, Room 2B10, Bethesda, MD 20892-4255, USA
Resource Type: Journal article
Publication Details: Mutation research, Vol.473(1), pp.121-136
Publisher: Elsevier B.V
DOI: 10.1016/S0027-5107(00)00143-3
PMID: 11166031
ISSN: 0027-5107
eISSN: 1873-135X
Language: English
Date published: 2001
Academic Unit: Pathology
Record Identifier: 9984083831702771

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