Induction of CD8+ regulatory T cells in the intestine by Heligmosomoides polygyrus infection

Ahmed Metwali; Tommy Setiawan; Arthur M Blum; Joseph Urban; David E Elliott; Long Hang; Joel V Weinstock

doi:10.1152/ajpgi.00409.2005

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Induction of CD8+ regulatory T cells in the intestine by Heligmosomoides polygyrus infection

Journal article

Open access

Peer reviewed

Induction of CD8+ regulatory T cells in the intestine by Heligmosomoides polygyrus infection

Ahmed Metwali, Tommy Setiawan, Arthur M Blum, Joseph Urban, David E Elliott, Long Hang and Joel V Weinstock

American journal of physiology: Gastrointestinal and liver physiology, Vol.291(2), pp.G253-259

08/2006

DOI: 10.1152/ajpgi.00409.2005

PMID: 16825660

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Abstract

This study determined whether Heligmosomoides polygyrus induces intestinal regulatory T cells. Splenic T cells proliferate strongly when cultured with anti-CD3 and antigen-presenting cells (APC). Lamina propria T cells from mice with H. polygyrus mixed with normal splenic T cells from uninfected mice inhibited proliferation over 90%. Lamina propria T cells from mice without H. polygyrus only modestly affected T cell proliferation. The worm-induced regulatory T cell was CD8+ and required splenic T cell contact to inhibit proliferation. The regulation also was IL-10 independent, but TAP-dependent, suggesting that it requires major histocompatibility complex (MHC) class I interaction. Additional studies employed mice with transgenic T cells that did not express functional TGF-beta receptors. The lamina propria T regulator inhibited proliferation of these transgenic T cells nearly 100%, suggesting that TGF-beta signaling via the T cell was not required. CD8+ T cells were needed for worms to reverse piroxicam-induced colitis in Rag mice (T and B cell deficient) reconstituted with IL-10-/- T cells. Thus H. polygyrus induces a regulatory CD8+ lamina propria T cell that inhibits T cell proliferation and that appears to have a role in control of colitis.

CD8-Positive T-Lymphocytes - pathology

Mice, Inbred C57BL

Intestines - immunology

Colitis - parasitology

Strongylida Infections - pathology

Lymphocyte Activation - immunology

Animals

Nematospiroides dubius

Strongylida Infections - immunology

Intestines - parasitology

Mice

CD8-Positive T-Lymphocytes - immunology

Colitis - immunology

Details

Title: Subtitle: Induction of CD8+ regulatory T cells in the intestine by Heligmosomoides polygyrus infection
Creators: Ahmed Metwali - Department of Internal Medicine, University of Iowa, Iowa City, USA
Tommy Setiawan
Arthur M Blum
Joseph Urban
David E Elliott
Long Hang
Joel V Weinstock
Resource Type: Journal article
Publication Details: American journal of physiology: Gastrointestinal and liver physiology, Vol.291(2), pp.G253-259
DOI: 10.1152/ajpgi.00409.2005
PMID: 16825660
NLM abbreviation: Am J Physiol Gastrointest Liver Physiol
ISSN: 0193-1857
eISSN: 1522-1547
Grant note: DK-25295 / NIDDK NIH HHS DK-38327 / NIDDK NIH HHS DK-58755 / NIDDK NIH HHS DK-07663 / NIDDK NIH HHS
Language: English
Date published: 08/2006
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984094539802771

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