Journal article
Induction of DNA single-strand breaks in human lymphocytes by low doses of γ-rays
International journal of radiation biology, Vol.68(5), pp.563-569
1995
DOI: 10.1080/09553009514551551
PMID: 7490506
Abstract
We hypothesized that very low doses of γ-rays have a genotoxic effect as measured by DNA single-strand breaks (ssbs). To test our hypothesis we used a newly developed alkaline microgel electrophoretic technique to quantitate DNA ssbs in freshly isolated human lymphocytes. An enhanced sensitivity to detect low levels of DNA damage was made possible by this method due to an additional step of DNA precipitation by ethanol in agarose microgels. We exposed lymphocytes from three subjects for 1 h at 4°C to 740 MBq of technetium 99-m at a distance of 20 cm (0·25 mGy), 10 cm (1·02 mGy), and 5 cm (4·08 mGy) from the source vial. Doses of 1·02 and 4·08 mGy induced a significant dose-dependent increase in DNA ssbs in human lymphocytes from three subjects, each examined on three different days.
Details
- Title: Subtitle
- Induction of DNA single-strand breaks in human lymphocytes by low doses of γ-rays
- Creators
- N. P SINGH - Univ. Washington, school medicine, dep. radiology, Seattle WA 98195-6560, United StatesM. M GRAHAM - Univ. Washington, school medicine, dep. radiology, Seattle WA 98195-6560, United StatesV SINGH - Univ. Washington, school medicine, dep. radiology, Seattle WA 98195-6560, United StatesA KHAN - Univ. Washington, school medicine, dep. radiology, Seattle WA 98195-6560, United States
- Resource Type
- Journal article
- Publication Details
- International journal of radiation biology, Vol.68(5), pp.563-569
- Publisher
- Taylor & Francis; London
- DOI
- 10.1080/09553009514551551
- PMID
- 7490506
- ISSN
- 0955-3002
- eISSN
- 1362-3095
- Language
- English
- Date published
- 1995
- Academic Unit
- Radiology; Radiation Oncology
- Record Identifier
- 9984047632302771
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