Journal article
Induction of Inflammasome-dependent Pyroptosis by Carbon Black Nanoparticles
The Journal of biological chemistry, Vol.286(24), pp.21844-21852
06/17/2011
DOI: 10.1074/jbc.M111.238519
PMCID: PMC3122239
PMID: 21525001
Abstract
Inhalation of nanoparticles has been implicated in respiratory morbidity and mortality. In particular, carbon black nanoparticles are found in many different environmental exposures. Macrophages take up inhaled nanoparticles and respond via release of inflammatory mediators and in some cases cell death. Based on new data, we propose that exposure of macrophages (both a macrophage cell line and primary human alveolar macrophages) to carbon black nanoparticles induces pyroptosis, an inflammasome-dependent form of cell death. Exposure of macrophages to carbon black nanoparticles resulted in inflammasome activation as defined by cleavage of caspase 1 to its active form and downstream IL-1β release. The cell death that occurred with carbon black nanoparticle exposure was identified as pyroptosis by the protective effect of a caspase 1 inhibitor and a pyroptosis inhibitor. These data demonstrate that carbon black nanoparticle exposure activates caspase 1, increases IL-1β release after LPS priming, and induces the proinflammatory cell death, pyroptosis. The identification of pyroptosis as a cellular response to carbon nanoparticle exposure is novel and relates to environmental and health impacts of carbon-based particulates.
Details
- Title: Subtitle
- Induction of Inflammasome-dependent Pyroptosis by Carbon Black Nanoparticles
- Creators
- Anna C. Reisetter - University of IowaLarissa V. Stebounova - University of IowaJonas Baltrusaitis - University of IowaLinda Powers - University of IowaAmit Gupta - University of IowaVicki H. Grassian - University of IowaMartha M. Monick - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.286(24), pp.21844-21852
- Publisher
- Elsevier Inc
- DOI
- 10.1074/jbc.M111.238519
- PMID
- 21525001
- PMCID
- PMC3122239
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 06/17/2011
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Chemistry; Chemical and Biochemical Engineering; Internal Medicine
- Record Identifier
- 9984622052302771
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