Induction of calcitonin gene-related peptide expression in rats by cortical spreading depression

Yan Wang; Anne E Tye; Junli Zhao; Dongqing Ma; Ann C Raddant; Fan Bu; Benjamin L Spector; Nolan K Winslow; Minyan Wang; Andrew F Russo

doi:10.1177/0333102416678388

Back

Induction of calcitonin gene-related peptide expression in rats by cortical spreading depression

Journal article

Open access

Peer reviewed

Induction of calcitonin gene-related peptide expression in rats by cortical spreading depression

Yan Wang, Anne E Tye, Junli Zhao, Dongqing Ma, Ann C Raddant, Fan Bu, Benjamin L Spector, Nolan K Winslow, Minyan Wang and Andrew F Russo

Cephalalgia, Vol.39(3), pp.333-341

03/2019

DOI: 10.1177/0333102416678388

PMCID: PMC5461204

PMID: 27919019

Files and links (1)

url

https://doi.org/10.1177/0333102416678388View

Published (Version of record) Open Access

Abstract

Objective The neuropeptide calcitonin gene-related peptide (CGRP) has now been established as a key player in migraine. However, the mechanisms underlying the reported elevation of CGRP in the serum and cerebrospinal fluid of some migraineurs are not known. A candidate mechanism is cortical spreading depression (CSD), which is associated with migraine with aura and traumatic brain injury. The aim of this study was to investigate whether CGRP gene expression may be induced by experimental CSD in the rat cerebral cortex. Methods CSD was induced by topical application of KCl and monitored using electrophysiological methods. Quantitative PCR and ELISA were used to measure CGRP mRNA and peptide levels in discrete ipsilateral and contralateral cortical regions of the rat brain 24 hours following CSD events and compared with sham treatments. Results The data show that multiple, but not single, CSD events significantly increase CGRP mRNA levels at 24 hours post-CSD in the ipsilateral rat cerebral cortex. Increased CGRP was observed in the ipsilateral frontal, motor, somatosensory, and visual cortices, but not the cingulate cortex, or contralateral cortices. CSD also induced CGRP peptide expression in the ipsilateral, but not contralateral, cortex. Conclusions Repeated CSD provides a mechanism for prolonged elevation of CGRP in the cerebral cortex, which may contribute to migraine and post-traumatic headache.

Details

Title: Subtitle: Induction of calcitonin gene-related peptide expression in rats by cortical spreading depression
Creators: Yan Wang - Centre for Neuroscience and
Anne E Tye - Department of Biological Sciences, Xi’an Jiaotong-Liverpool University (XJTLU), SIP, Suzhou 215123, China
Junli Zhao - Centre for Neuroscience and
Dongqing Ma - Centre for Neuroscience and, Neuroscience Program, Departments of
Ann C Raddant - Molecular Physiology and Biophysics, and
Fan Bu - Centre for Neuroscience and, Neuroscience Program, Departments of
Benjamin L Spector - Molecular Physiology and Biophysics, and
Nolan K Winslow - Molecular Physiology and Biophysics, and
Minyan Wang - Centre for Neuroscience and, Neuroscience Program, Departments of
Andrew F Russo - Department of Biological Sciences, Xi’an Jiaotong-Liverpool University (XJTLU), SIP, Suzhou 215123, China, Molecular Physiology and Biophysics, and, Neurology, University of Iowa, Iowa City, IA 52242, USA;, Veterans Affairs Medical Center, Iowa City, IA 52246, USA
Resource Type: Journal article
Publication Details: Cephalalgia, Vol.39(3), pp.333-341
DOI: 10.1177/0333102416678388
PMID: 27919019
PMCID: PMC5461204
NLM abbreviation: Cephalalgia
ISSN: 0333-1024
eISSN: 1468-2982
Language: English
Date published: 03/2019
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center
Record Identifier: 9984013920502771

Metrics

12 Record Views

43 Times Cited - Web of Science