Journal article
Induction of calcium currents by the expression of the α1-subunit of the dihydropyridine receptor from skeletal muscle
Nature (London), Vol.340(6230), pp.233-236
07/1989
DOI: 10.1038/340233a0
Abstract
THE dihydropyridine (DHP) receptor purified from skeletal muscle comprises five protein subunits (α1, α2, β, γ and δ) and produces Ca2+ currents that are blocked by DHPs1. Cloning of the α1- and α2-subunits2,3, the former affinity-labelled by DHP, has shown that the α1-subunit is expressed in skeletal muscle alone, whereas the α2- and δ-subunits are also expressed in othertissues2,4,5. Although the transient expression of the α1-subunit in myoblasts from dysgenic mice (but not in oocytes) has been demonstrated6, the use of these expression systems to determine the function of the α1-subunit is complicated by the presence of endogenous Ca2+ currents7,8, which may reflect the constitutive expression of proteins similar to the α2-, β-, γ- and/or γ-subunits. We therefore selected a cell line which has no Ca2+ currents or α2-subunit, and probably no γ-subunit for stable transformation with complementary DNA of the α1-subunit. The transformed cells express DHP-sensitive, voltage-gated Ca2+ channels, indicating that the minimum structure of these channels is at most an α1βγ complex and possibly an α1-subunit alone.
Details
- Title: Subtitle
- Induction of calcium currents by the expression of the α1-subunit of the dihydropyridine receptor from skeletal muscle
- Creators
- Edward Perez–ReyesHaeyoung S KimAntonio E LacerdaWilliam HorneXiangyang WeiDavid RampeKevin P CampbellArthur M BrownLutz Birnbaumer
- Resource Type
- Journal article
- Publication Details
- Nature (London), Vol.340(6230), pp.233-236
- DOI
- 10.1038/340233a0
- ISSN
- 0028-0836
- eISSN
- 1476-4687
- Language
- English
- Date published
- 07/1989
- Academic Unit
- Molecular Physiology and Biophysics; Neurology; Iowa Neuroscience Institute
- Record Identifier
- 9984020847802771
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