Induction of carcinoma cell migration on vitronectin by NF-kappa B-dependent gene expression

Mayra Yebra; Edward J Filardo; Evelyn M Bayna; Ei Kawahara; Jurgen C Becker; David A Cheresh

doi:10.1091/mbc.6.7.841

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Induction of carcinoma cell migration on vitronectin by NF-kappa B-dependent gene expression

Journal article

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Induction of carcinoma cell migration on vitronectin by NF-kappa B-dependent gene expression

Mayra Yebra, Edward J Filardo, Evelyn M Bayna, Ei Kawahara, Jurgen C Becker and David A Cheresh

Molecular biology of the cell, Vol.6(7), pp.841-850

07/1995

DOI: 10.1091/mbc.6.7.841

PMID: 7579698

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https://www.ncbi.nlm.nih.gov/pmc/articles/301244View

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Abstract

Integrin alpha v beta 5 promotes FG carcinoma cell adhesion to vitronectin yet requires protein kinase C (PKC) activation for migration on this ligand. Here we report that this PKC-dependent cell motility event requires NF-kappaB-dependent transcription. Specifically, a component within nuclear extracts prepared from PKC-stimulated FG cells exhibited a significant increase in binding activity to a synthetic oligonucleotide containing a consensus kappa B sequence. These nuclear DNA-binding complexes were shown to be comprised of p65 and p50 NF-kappaB/rel family members and appeared functionally active because they promoted transcription of a reporter construct containing a kappa B site. The NF-kappa B activation event was directly linked to the alpha v beta 5 motility response because the NF-kappa B-binding oligonucleotide, when introduced into FG cells, inhibited cell migration on vitronectin but not on collagen and had no effect on cell adhesion to either ligand. These results suggest that the detected DNA-binding complexes interact with kappa B transcriptional elements to regulate gene expression required for alpha v beta 5-dependent cell motility on vitronectin.

Details

Title: Subtitle: Induction of carcinoma cell migration on vitronectin by NF-kappa B-dependent gene expression
Creators: Mayra Yebra - Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
Edward J Filardo - Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
Evelyn M Bayna - Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
Ei Kawahara - Kanazawa University
Jurgen C Becker - Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
David A Cheresh - Department of Immunology, Scripps Research Institute, La Jolla, California 92037, USA
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.6(7), pp.841-850
DOI: 10.1091/mbc.6.7.841
PMID: 7579698
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology
Language: English
Date published: 07/1995
Academic Unit: Pharmaceutical Sciences and Experimental Therapeutics; Surgery; Internal Medicine
Record Identifier: 9984051735502771

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