Journal article
Induction of chromosomal aberrations by the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl in cultured human lymphocytes
Mutagenesis, Vol.15(5), pp.375-378
09/2000
DOI: 10.1093/mutage/15.5.375
PMID: 10970441
Abstract
The genotoxicity of the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl (complex R) in cultured human lymphocytes was studied using the chromosome aberrations (CAs) assay. Lymphocyte cultures were initiated from two adult healthy non-smoking male volunteers and were exposed to the complex for a duration of 3 or 20 h prior to cell collection. The reduction in mitotic indices (MI) and the induction of CAs represented the toxic and clastogenic effects of the different treatments, respectively. Complex R proved to be an intermediate toxic clastogen with a MI50 of 1.0 μg/ml and a minimum positive dose (MPD) of 0.1 μg/ml. Like bleomycin, complex R exerted its clastogenic effects without the need for metabolic activation and induced CAs of all types in lymphocytes treated in the G2 and late S phases and, therefore, can be considered a radiomimetic. In addition, it induced more total CAs of chromatid-type than of chromosome-type. The reduction in the frequencies of CAs following the 20 h treatment as compared with those induced following the 3 h treatments indicated that human lymphocytes in the presence of complex R can partially tolerate the lesions involved in CA production. Based on the biological effects of complex R and the similarities between its functional group and that of bleomycin, possible mechanisms for complex R genotoxicity are discussed.
Details
- Title: Subtitle
- Induction of chromosomal aberrations by the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl in cultured human lymphocytes
- Creators
- May F SadiqMukarram H Zaghal - Department of Chemistry, Faculty of Sciences, Yarmouk University, Irbid andHatem E El-Shanti - Department of Paediatrics and Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan
- Resource Type
- Journal article
- Publication Details
- Mutagenesis, Vol.15(5), pp.375-378
- Publisher
- Oxford University Press
- DOI
- 10.1093/mutage/15.5.375
- PMID
- 10970441
- ISSN
- 0267-8357
- eISSN
- 1464-3804
- Language
- English
- Date published
- 09/2000
- Academic Unit
- Stead Family Department of Pediatrics; Medical Genetics and Genomics
- Record Identifier
- 9984093495502771
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