Induction of neutrophil extracellular traps by Campylobacter jejuni

Sean Callahan; Ryan S Doster; Joseph W Jackson; Brittni R Kelley; Jennifer A Gaddy; Jeremiah G Johnson

doi:10.1111/cmi.13210

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Induction of neutrophil extracellular traps by Campylobacter jejuni

Journal article

Open access

Peer reviewed

Induction of neutrophil extracellular traps by Campylobacter jejuni

Sean Callahan, Ryan S Doster, Joseph W Jackson, Brittni R Kelley, Jennifer A Gaddy and Jeremiah G Johnson

Cellular microbiology, Vol.22(8), pp.e13210-n/a

08/2020

DOI: 10.1111/cmi.13210

PMCID: PMC7354212

PMID: 32329205

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Abstract

Campylobacter jejuni is the leading cause of bacterial-derived gastroenteritis worldwide and can lead to several post-infectious inflammatory disorders. Despite the prevalence and health impacts of the bacterium, interactions between the host innate immune system and C. jejuni remain poorly understood. To expand on earlier work demonstrating that neutrophils traffic to the site of infection in an animal model of campylobacteriosis, we identified significant increases in several predominantly neutrophil-derived proteins in the faeces of C. jejuni-infected patients, including lipocalin-2, myeloperoxidase and neutrophil elastase. In addition to demonstrating that these proteins significantly inhibited C. jejuni growth, we determined they are released during formation of C. jejuni-induced neutrophil extracellular traps (NETs). Using quantitative and qualitative methods, we found that purified human neutrophils are activated by C. jejuni and exhibit signatures of NET generation, including presence of protein arginine deiminase-4, histone citrullination, myeloperoxidase, neutrophil elastase release and DNA extrusion. Production of NETs correlated with C. jejuni phagocytosis/endocytosis and invasion of neutrophils suggesting that host- and bacterial-mediated activities are responsible for NET induction. Further, NET-like structures were observed within intestinal tissue of C. jejuni-infected ferrets. Finally, induction of NETs significantly increased human colonocyte cytotoxicity, indicating that NET formation during C. jejuni infection may contribute to observed tissue pathology. These findings provide further understanding of C. jejuni-neutrophil interactions and inflammatory responses during campylobacteriosis.

Animals

Campylobacter Infections - immunology

Campylobacter Infections - microbiology

Campylobacter jejuni - immunology

Campylobacter jejuni - physiology

Cells, Cultured

Colon - cytology

Colon - microbiology

Colon - pathology

Extracellular Traps - immunology

Extracellular Traps - microbiology

Feces - chemistry

Ferrets

Host Microbial Interactions - immunology

Humans

Inflammation

Leukocyte Elastase - metabolism

Male

Neutrophils - chemistry

Neutrophils - immunology

Neutrophils - microbiology

Phagocytosis

Details

Title: Subtitle: Induction of neutrophil extracellular traps by Campylobacter jejuni
Creators: Sean Callahan - University of Tennessee at Knoxville
Ryan S Doster - Vanderbilt University Medical Center
Joseph W Jackson - University of Tennessee at Knoxville
Brittni R Kelley - University of Tennessee at Knoxville
Jennifer A Gaddy - Vanderbilt University Medical Center
Jeremiah G Johnson - University of Tennessee at Knoxville
Resource Type: Journal article
Publication Details: Cellular microbiology, Vol.22(8), pp.e13210-n/a
DOI: 10.1111/cmi.13210
PMID: 32329205
PMCID: PMC7354212
NLM abbreviation: Cell Microbiol
ISSN: 1462-5814
eISSN: 1462-5822
Grant note: R01 HD090061 / NICHD NIH HHS P30 DK058404 / NIDDK NIH HHS IK2 BX001701 / BLRD VA UL1 RR024975 / NCRR NIH HHS P30 DK020593 / NIDDK NIH HHS R01 AI134036 / NIH HHS R01 AI134036 / NIAID NIH HHS P30 DK058404 / NIH HHS UL1 TR000445 / NCATS NIH HHS P30 CA068485 / NCI NIH HHS P30 EY008126 / NEI NIH HHS T32 CA082084 / NCI NIH HHS U24 DK059637 / NIDDK NIH HHS K08 AI151100 / NIAID NIH HHS U01 TR002398 / NCATS NIH HHS
Language: English
Date published: 08/2020
Academic Unit: Microbiology and Immunology
Record Identifier: 9984696570302771

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