Journal article
Induction of specific cell-mediated immunity in mice by oral immunization with Salmonella expressing Onchocerca volvulus glutathione S-transferase
Vaccine, Vol.17(1), pp.31-39
1999
DOI: 10.1016/S0264-410X(98)00147-9
PMID: 10078605
Abstract
Cellular and humoral immune responses of mice to
Onchocerca volvulus glutathione
S-transferase (OvGST) presented via in vivo expression in attenuated
Salmonella typhimurium were examined and compared with the same antigen administered by subcutaneous injection with Freund's adjuvant. After infection with recombinant
S. typhimurium, maximal numbers of bacteria were recovered from the mesenteric lymph nodes and spleens during the second week postinfection. By weeks 3–4, bacteria were absent from these tissues. Splenocytes from mice infected with
S. typhimurium expressing OvGST showed significant and specific proliferative responses to OvGST, whereas the non-recombinant
S. typhimurium controls and those which received the antigen by subcutaneous injection with Freund's adjuvant did not. Mice infected with recombinant
S. typhimurium had elevated IFN-
γ levels over non-recombinant
S. typhimurium and placebo controls, but IL-4 and IL-5 levels were low and did not differ significantly between these groups. Antibody responses to OvGST antigen expressed by a recombinant
Salmonella vaccine or delivered in a purified form with Freund's adjuvant were moderate to high. These data suggest that
Salmonella can be used as a vaccine delivery vector that induces specific cellular and humoral immune responses to
Onchocerca volvulus antigens. This is the first report to describe the successful application of a filarial antigen in a live-vector delivery system as well as the first recombinant based filarial vaccine to elicit a cellular immune response similar to that described for putative immune endemics.
Details
- Title: Subtitle
- Induction of specific cell-mediated immunity in mice by oral immunization with Salmonella expressing Onchocerca volvulus glutathione S-transferase
- Creators
- Julian Catmull - Departments of Biochemistry, University of Iowa, Iowa City, IA 52242, USAMary E Wilson - Department of Internal Medicine and Microbiology, University of Iowa, Iowa City, IA 52242, USALouis V Kirchhoff - The Veterans' Affairs Medical Center, Iowa City, IA 52242, USAAhmed Metwali - Department of Internal Medicine and Microbiology, University of Iowa, Iowa City, IA 52242, USAJohn E Donelson - Howard Hughes Medical Institute, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Vaccine, Vol.17(1), pp.31-39
- DOI
- 10.1016/S0264-410X(98)00147-9
- PMID
- 10078605
- NLM abbreviation
- Vaccine
- ISSN
- 0264-410X
- eISSN
- 1873-2518
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 1999
- Academic Unit
- Psychiatry; Microbiology and Immunology; Infectious Diseases; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984002392402771
Metrics
15 Record Views