Journal article
Inefficient bypass of an abasic site by DNA polymerase eta
The Journal of biological chemistry, Vol.276(9), pp.6861-6866
03/02/2001
DOI: 10.1074/jbc.M008021200
PMID: 11106652
Abstract
DNA polymerase eta (Pol eta) bypasses a cis-syn thymine-thymine dimer efficiently and accurately, and inactivation of Pol eta in humans results in the cancer-prone syndrome, the variant form of xeroderma pigmentosum. Also, Pol eta bypasses the 8-oxoguanine lesion efficiently by predominantly inserting a C opposite this lesion, and it bypasses the O(6)-methylguanine lesion by inserting a C or a T. To further assess the range of DNA lesions tolerated by Pol eta, here we examine the bypass of an abasic site, a prototypical noninstructional lesion. Steady-state kinetic analyses show that both yeast and human Pol eta are very inefficient in both inserting a nucleotide opposite an abasic site and in extending from the nucleotide inserted. Hence, Pol eta bypasses this lesion extremely poorly. These results suggest that Pol eta requires the presence of template bases opposite both the incoming nucleotide and the primer terminus to catalyze efficient nucleotide incorporation.
Details
- Title: Subtitle
- Inefficient bypass of an abasic site by DNA polymerase eta
- Creators
- Lajos Haracska - Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1061, USAM Todd WashingtonSatya PrakashLouise Prakash
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.276(9), pp.6861-6866
- DOI
- 10.1074/jbc.M008021200
- PMID
- 11106652
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- United States
- Grant note
- GM19261 / NIGMS NIH HHS
- Language
- English
- Date published
- 03/02/2001
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024561702771
Metrics
19 Record Views