Journal article
Infection and Activation of Human Neutrophils with Fluorescent Leishmania infantum
Journal of Immunological Techniques in Infectious Diseases, Vol.5(3), 1000146
2016
DOI: 10.4172/2329-9541.1000146
PMCID: PMC6205212
PMID: 30381805
Abstract
Neutrophils (PMNs) are recruited in high numbers to sites of host infection by the protozoan parasites of the genus Leishmania. Although PMNs are capable of phagocytizing Leishmania parasites and are potent producers of antimicrobial compounds including reactive oxygen species (ROS), they are unable to control the establishment of infection. Prior studies document production of ROS in isolated PMNs incubated with Leishmania under conditions allowing phagocytosis, however without a measure of single cells’ responses it cannot be discerned whether PMN activation and ROS production is suppressed or ineffective in the cells that internalize the parasite. To address these interactions, we engineered a strain of fluorescent, mCherry-expressing Leishmania infantum (mCherry-Li).
Details
- Title: Subtitle
- Infection and Activation of Human Neutrophils with Fluorescent Leishmania infantum
- Creators
- R E DavisC J ThalhoferM E Wilson
- Resource Type
- Journal article
- Publication Details
- Journal of Immunological Techniques in Infectious Diseases, Vol.5(3), 1000146
- DOI
- 10.4172/2329-9541.1000146
- PMID
- 30381805
- PMCID
- PMC6205212
- NLM abbreviation
- J Immunol Tech Infect Dis
- ISSN
- 2329-9541
- eISSN
- 2329-9541
- Language
- English
- Date published
- 2016
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001112902771
Metrics
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