Infection of Human Airway Epithelia with H1N1, H2N2, and H3N2 Influenza A Virus Strains

Vladimir A Slepushkin; Patrick D Staber; Guoshun Wang; Paul B McCray; Beverly L Davidson

doi:10.1006/mthe.2001.0277

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Infection of Human Airway Epithelia with H1N1, H2N2, and H3N2 Influenza A Virus Strains

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Infection of Human Airway Epithelia with H1N1, H2N2, and H3N2 Influenza A Virus Strains

Vladimir A Slepushkin, Patrick D Staber, Guoshun Wang, Paul B McCray and Beverly L Davidson

Molecular therapy, Vol.3(3), pp.395-402

03/2001

DOI: 10.1006/mthe.2001.0277

PMCID: PMC7106098

PMID: 11273782

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Abstract

Three subtypes of influenza A virus cause human disease: H1N1, H2N2, and H3N2. Although all result in respiratory illness, little is known about how these subtypes infect differentiated airway epithelia. Therefore, we assayed A/PR/8/34 (H1N1), A/Japan/305/57 (H2N2), and X31 (H3N2) influenza virus strains for binding and infection on fully differentiated primary cultures of airway epithelia isolated from human bronchus, grown on semiporous filters at an air–liquid interface. In this model system, viral infectivity was highest when virus was applied to the apical versus the basolateral surface; Japan was most infectious, followed by PR8. The X31 strain showed very low levels of infectivity. Confocal microscopy and fluorescence-resonance energy transfer studies indicated that Japan virus could enter and fuse with cellular membranes, while infection with X31 virions was greatly inhibited. Japan virus could also productively infect human trachea explant tissues. These data show that influenza viruses with SAα2,3Gal binding specificity, like Japan, productively infect differentiated human airway epithelia from the apical surface. These data are important to consider in the development of pseudotyped recombinant viral vectors for gene transfer to human airway epithelia for gene therapy.

gene therapy

pseudotype

Details

Title: Subtitle: Infection of Human Airway Epithelia with H1N1, H2N2, and H3N2 Influenza A Virus Strains
Creators: Vladimir A Slepushkin - Program in Gene Therapy, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa, 52242
Patrick D Staber - Program in Gene Therapy, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa, 52242
Guoshun Wang - Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa, 52242
Paul B McCray - Program in Gene Therapy, Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa, 52242
Beverly L Davidson - Program in Gene Therapy, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa, 52242
Resource Type: Journal article
Publication Details: Molecular therapy, Vol.3(3), pp.395-402
DOI: 10.1006/mthe.2001.0277
PMID: 11273782
PMCID: PMC7106098
NLM abbreviation: Mol Ther
ISSN: 1525-0016
eISSN: 1525-0024
Publisher: Elsevier Inc
Language: English
Date published: 03/2001
Academic Unit: Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
Record Identifier: 9984093359702771

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