Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth

Saulo Geraldeli; Yalan Li; Mary M.B Hogan; Leo S Tjaderhane; David H Pashley; Teresa A Morgan; M. Bridget Zimmerman; Kim A Brogden

doi:10.1016/j.archoralbio.2011.08.012

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Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth

Journal article

Peer reviewed

Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth

Saulo Geraldeli, Yalan Li, Mary M.B Hogan, Leo S Tjaderhane, David H Pashley, Teresa A Morgan, M. Bridget Zimmerman and Kim A Brogden

Archives of oral biology, Vol.57(3), pp.264-270

03/2012

DOI: 10.1016/j.archoralbio.2011.08.012

PMID: 21930261

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Abstract

Chemokines and cytokines may occur in dentinal fluids in response to local infection and inflammation. To test this hypothesis, we assessed the presence and concentration of inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. Freshly extracted sound, carious, and restored molars were trimmed through the enamel to expose the underlying dentine, etched with 35% phosphoric acid, and rinsed. Fluid was extracted from the coronal occlusal dentine of these trimmed teeth by centrifugation at 2750×g for 30min. When assessed by MALDI-TOF, fluid extracted from the coronal occlusal dentine from 16 molars contained at least 117 peaks with different masses suggesting that this fluid was rich with molecules within the appropriate mass range of potential mediators. Indeed, when assessed for chemokines and cytokines, fluid extracted from the coronal occlusal dentine from 25 extracted molars with caries lesions, 10 extracted restored molars with occlusal amalgam, and 77 extracted sound molars contained IL-1β, TNF-α, IL-6, IL-8, IL-12(p70), and IL-10. A significant elevation was found for TNF-α (p=0.041) in extracted fluid from teeth restored with amalgam fillings. Overall, fluid extracted from the coronal occlusal dentine of trimmed teeth may be useful in identifying proteins and other molecules in dentine and pulpal fluids and determining their role as mediators in the pathogenesis of oral infection and inflammation.

Cytokines

MALDI-TOF

Chemokines

Dentinal fluid

Details

Title: Subtitle: Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth
Creators: Saulo Geraldeli - Restorative Dental Sciences Operative Division, College of Dentistry, University of Florida, Gainesville, FL 32610, USA
Yalan Li - Proteomics Facility, College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
Mary M.B Hogan - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
Leo S Tjaderhane - Institute of Dentistry, University of Oulu, Oulu, Finland
David H Pashley - College of Dental Medicine, Georgia Health Sciences University, Augusta, GA 30912, USA
Teresa A Morgan - The Department of Oral & Maxillofacial Surgery, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
M. Bridget Zimmerman - Biostatistics Consulting Center, Department of Biostatistics, University of Iowa, 200 Hawkins Drive, C22-E GH, Iowa City, IA 52242, USA
Kim A Brogden - Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Archives of oral biology, Vol.57(3), pp.264-270
DOI: 10.1016/j.archoralbio.2011.08.012
PMID: 21930261
NLM abbreviation: Arch Oral Biol
ISSN: 0003-9969
eISSN: 1879-1506
Publisher: Elsevier Ltd
Grant note: name: University of Iowa, College of Dentistry Seed money; name: NIH/NIDCR, award: R01 DE014390
Language: English
Date published: 03/2012
Academic Unit: Biostatistics; Oral and Maxillofacial Surgery; Dental Research; Hospital Dentistry; Periodontics
Record Identifier: 9983997304002771

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