Inflammatory protein profiling of pancreatic cyst fluid using EUS-FNA in tandem with cytokine microarray differentiates between branch duct IPMN and inflammatory cysts

Linda S Lee; Peter A Banks; Andrew M Bellizzi; Nisha I Sainani; Vivek Kadiyala; Shadeah Suleiman; Darwin L Conwell; Joao A Paulo

doi:10.1016/j.jim.2012.05.018

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Inflammatory protein profiling of pancreatic cyst fluid using EUS-FNA in tandem with cytokine microarray differentiates between branch duct IPMN and inflammatory cysts

Journal article

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Inflammatory protein profiling of pancreatic cyst fluid using EUS-FNA in tandem with cytokine microarray differentiates between branch duct IPMN and inflammatory cysts

Linda S Lee, Peter A Banks, Andrew M Bellizzi, Nisha I Sainani, Vivek Kadiyala, Shadeah Suleiman, Darwin L Conwell and Joao A Paulo

Journal of immunological methods, Vol.382(1-2), pp.142-149

08/31/2012

DOI: 10.1016/j.jim.2012.05.018

PMCID: PMC4509568

PMID: 22683544

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Abstract

Diagnosis of pancreatic cystic neoplasms remains problematic. We hypothesize that inflammatory mediator proteins in pancreatic cyst fluid can differentiate branch duct intraductal papillary mucinous neoplasms (BD-IPMNs) and pancreatic inflammatory cysts. We aim to 1) detect inflammatory mediator proteins (IMPs) using a multiplexed IMP-targeted microarray in pancreatic cyst fluid obtained during endoscopic ultrasound fine needle aspiration (EUS-FNA) and 2) compare IMP profiles in pancreatic cyst fluid from BD-IPMNs and inflammatory cysts. Pancreatic cyst fluid from ten patients (5 BD-IPMN and 5 inflammatory cysts) was obtained by EUS-FNA and analyzed directly with a multiplexed microarray assay to determine concentrations of 89 IMPs. Statistical analysis was performed using non-parametric methods. Eighty-three of the 89 assayed IMPs were detected in at least one of the 10 patient samples. Seven IMPs were detected in BD-IPMN but not inflammatory cysts, while eleven IMPs were identified in inflammatory cysts but not BD-IPMN. Notably, granulocyte–macrophage colony-stimulating factor (GM-CSF) expression was present in all five inflammatory cyst samples. Hepatocyte growth factor (HGF) was present in significantly higher concentrations in inflammatory cysts compared to BD-IPMN. Our exploratory analysis reveals that GM-CSF and HGF in EUS-FNA-collected pancreatic cyst fluid can distinguish between BD-IPMN and inflammatory cyst. Coupling microarray molecular techniques to EUS-FNA may represent a major step forward to our understanding complex pancreatic disease. [Display omitted] ► EUS-FNA-sampled pancreatic cystic neoplasms are analyzed using a cytokine microarray. ► Seven IMPs were detected in BD-IPMN, but not in inflammatory cysts. ► Eleven IMPs were detected in inflammatory cysts, but not in BD-IMPNs. ► GM-CSF and HGF in pancreatic cyst fluid distinguishes BD-IPMN and inflammatory cysts. ► Coupling microarrays to EUS-FNA is a major step in understanding pancreatic disease.

Biomarker

IPMN

Endoscopic ultrasound fine needle aspiration

Pancreas cyst

Pseudocyst

Cytokine

Details

Title: Subtitle: Inflammatory protein profiling of pancreatic cyst fluid using EUS-FNA in tandem with cytokine microarray differentiates between branch duct IPMN and inflammatory cysts
Creators: Linda S Lee - Center for Pancreatic Disease, Division of Gastroenterology Hepatology and Endoscopy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States
Peter A Banks - Center for Pancreatic Disease, Division of Gastroenterology Hepatology and Endoscopy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States
Andrew M Bellizzi - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA, United States
Nisha I Sainani - Department of Radiology, Brigham and Women's Hospital, Boston, MA, United States
Vivek Kadiyala - Center for Pancreatic Disease, Division of Gastroenterology Hepatology and Endoscopy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States
Shadeah Suleiman - Center for Pancreatic Disease, Division of Gastroenterology Hepatology and Endoscopy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States
Darwin L Conwell - Center for Pancreatic Disease, Division of Gastroenterology Hepatology and Endoscopy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States
Joao A Paulo - Center for Pancreatic Disease, Division of Gastroenterology Hepatology and Endoscopy, Brigham and Women's Hospital and Department of Medicine, Harvard Medical School, Boston, MA, United States
Resource Type: Journal article
Publication Details: Journal of immunological methods, Vol.382(1-2), pp.142-149
DOI: 10.1016/j.jim.2012.05.018
PMID: 22683544
PMCID: PMC4509568
NLM abbreviation: J Immunol Methods
ISSN: 0022-1759
eISSN: 1872-7905
Publisher: Elsevier B.V
Grant note: name: American College of Gastroenterology Clinical Research Award; name: NIH NIDDK NRSA Fellowship, award: 1F32 DK085835-01A1; name: Harvard Digestive Diseases Center, award: 5 P30 DK034854-24; name: NIH NIDDK, award: 1R21 DK081703-01A2
Language: English
Date published: 08/31/2012
Academic Unit: Pathology; Cardiovascular Medicine; Internal Medicine
Record Identifier: 9984047682002771

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