Influence of 45S5 bioactive glass on the bonding properties to molar incisor hypomineralization-affected enamel: A 1-year in vitro evaluation

PHA Moreira; M N Correia; MWC Ferreira; ABB Moreira; V P Feitosa; M Jose-Burbano; AFM Cardenas; A D Loguercio; FSF Siqueira

doi:10.1016/j.jdent.2025.106211

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Influence of 45S5 bioactive glass on the bonding properties to molar incisor hypomineralization-affected enamel: A 1-year in vitro evaluation

Journal article

Peer reviewed

Influence of 45S5 bioactive glass on the bonding properties to molar incisor hypomineralization-affected enamel: A 1-year in vitro evaluation

PHA Moreira, M N Correia, MWC Ferreira, ABB Moreira, V P Feitosa, M Jose-Burbano, AFM Cardenas, A D Loguercio and FSF Siqueira

Journal of dentistry, Vol.164, 106211

01/2026

DOI: 10.1016/j.jdent.2025.106211

PMID: 41198014

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Abstract

Objective To evaluate the effect of a two-step, self-etch adhesive containing 45S5 bioactive glass on the microshear bond strength (µSBS) to enamel and on the resin-enamel interface by energy-dispersive X-ray spectrometry analysis (EDX-SEM), both immediately and after one year. In addition, to assess the in-situ degree of conversion (DC) at the enamel-adhesive interface formed between sound and molar incisor hypomineralization-affected enamel (MIH). Methods Eighty human molars were allocated into eight experimental groups according to: 1) enamel surface (sound [SD] and MIH-affected [MIH]); 2) adhesive (Clearfil SE Bond adhesive containing 0 % or 10 % of 45S5 bioactive glass); and 3) storage time (immediate or after one year of water storage). Following specimens’ preparation, resin-enamel cylinders were tested for µSBS (0.5 mm/min) or analysed by EDX-SEM. Resin-enamel slices were prepared to assess the in-situ DC. μSBS and DC data were analysed using three-way and two-way ANOVA, respectively, following by Tukey’s post-hoc test (α = 0.05) The resin–enamel interface was qualitatively analysed using EDX-SEM. Results Clearfil SE Bond achieved significantly higher μSBS and DC values on sound enamel compared to MIH-affected enamel (p < 0.0000001). The incorporation of 45S5 bioactive glass significantly increased μSBS without affecting in-situ DC values in both sound and MIH-affected enamel, at both immediate and 1-year evaluations (p < 0.0000001). When Clearfil SE Bond containing 45S5 was applied, EDX-SEM revealed the presence of calcium, phosphorus, and silicon at the resin–enamel interface, which remained stable after one year of storage, regardless of enamel condition. Conclusion The inclusion of 45S5 bioctive glass in a two-step self-etch adhesive significantly improved μSBS without affecting in-situ DC values, in both sound and MIH-affected enamel. This beneficial effect, maintained after one year of water storage, was associated with the chemical stability and sustained presence of calcium, phosphorus, and silicon at the resin–enamel interface.

Bioactive glass

Degree of conversion

Microshear bonding strength

Molar incisor hypomineralization

Self-etch

Details

Title: Subtitle: Influence of 45S5 bioactive glass on the bonding properties to molar incisor hypomineralization-affected enamel: A 1-year in vitro evaluation
Creators: PHA Moreira - Universidade Estadual de Ponta Grossa
M N Correia - Centro Universitário do Maranhão
MWC Ferreira - Universidade Estadual de Ponta Grossa
ABB Moreira - Universidade Federal do Maranhão
V P Feitosa - University of Iowa
M Jose-Burbano - Universidad de los Hemisferios
AFM Cardenas - Centro Universitário do Maranhão
A D Loguercio - Universidade Estadual de Ponta Grossa
FSF Siqueira - Universidade Federal do Maranhão
Resource Type: Journal article
Publication Details: Journal of dentistry, Vol.164, 106211
DOI: 10.1016/j.jdent.2025.106211
PMID: 41198014
NLM abbreviation: J Dent
ISSN: 0300-5712
eISSN: 1879-176X
Publisher: Elsevier Ltd
Grant note: State Foundation of Sup-port to Research, Scientific and Technological Development of Maranhao (FAPEMA) from the State Government of Maranhao BrazilNational Council for Scientific and Technological Development (CNPq): 304444/2025-1 Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES): 001
This study was partially supported by the State Foundation of Sup-port to Research, Scientific and Technological Development of Maranhao (FAPEMA) from the State Government of Maranhao Brazil and National Council for Scientific and Technological Development (CNPq) under grant 304444/2025-1 and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) - Finance Code 001.
Language: English
Electronic publication date: 11/04/2025
Date published: 01/2026
Academic Unit: Operative Dentistry
Record Identifier: 9985024141602771

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