Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Ana Carolina Ramos; Cecília Godoy Carvalhaes; Jhonatha Rodrigo Cordeiro-Moura; Anna Carolina Rockstroh; Antonia Maria Oliveira Machado; Ana Cristina Gales

doi:10.1128/JCM.00749-16

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Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Journal article

Open access

Peer reviewed

Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Ana Carolina Ramos, Cecília Godoy Carvalhaes, Jhonatha Rodrigo Cordeiro-Moura, Anna Carolina Rockstroh, Antonia Maria Oliveira Machado and Ana Cristina Gales

Journal of clinical microbiology, Vol.54(7), pp.1896-1898

07/2016

DOI: 10.1128/JCM.00749-16

PMCID: PMC4922090

PMID: 27076665

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https://doi.org/10.1128/JCM.00749-16View

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Abstract

In this study, we evaluated the influence of distinct bacterial growth media on detection of carbapenemase hydrolysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. False-negative results were observed for OXA-25-, OXA-26-, and OXA-72-producing Acinetobacter baumannii isolates grown on MacConkey agar medium. The other culture media showed 100% sensitivity and 100% specificity for detecting carbapenemase.

Acinetobacter baumannii - enzymology

Bacterial Proteins - analysis

beta-Lactamases - analysis

Carbapenems - metabolism

Culture Media - chemistry

False Negative Reactions

Hydrolysis

Sensitivity and Specificity

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

Details

Title: Subtitle: Influence of Culture Media on Detection of Carbapenem Hydrolysis by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Creators: Ana Carolina Ramos - Universidade Federal de São Paulo
Cecília Godoy Carvalhaes - Universidade Federal de São Paulo
Jhonatha Rodrigo Cordeiro-Moura - Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo, São Paulo, SP, Brazil
Anna Carolina Rockstroh - BioMérieux
Antonia Maria Oliveira Machado - Universidade Federal de São Paulo
Ana Cristina Gales - Universidade Federal de São Paulo
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.54(7), pp.1896-1898
DOI: 10.1128/JCM.00749-16
PMID: 27076665
PMCID: PMC4922090
ISSN: 0095-1137
eISSN: 1098-660X
Language: English
Date published: 07/2016
Academic Unit: Pathology
Record Identifier: 9984185166502771

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