Journal article
Influence of Substrate Stiffness on iPSC-Derived Retinal Pigmented Epithelial Cells
Stem cells translational medicine, Vol.13(6), pp.582-592
06/2024
DOI: 10.1093/stcltm/szae022
PMCID: PMC11165161
PMID: 38560893
Abstract
Retinal degenerative diseases are a major cause of blindness involving the dysfunction of photoreceptors, retinal pigmented epithelium (RPE), or both. A promising treatment approach involves replacing these cells via surgical transplantation, and previous work has shown that cell delivery scaffolds are vital to ensure sufficient cell survival. Thus, identifying scaffold properties that are conducive to cell viability and maturation (such as suitable material and mechanical properties) is critical to ensuring a successful treatment approach. In this study, we investigated the effect of scaffold stiffness on human RPE attachment, survival, and differentiation, comparing immortalized (ARPE-19) and stem cell-derived RPE (iRPE) cells. Polydimethylsiloxane was used as a model polymer substrate, and varying stiffness (~12 to 800 kPa) was achieved by modulating the cross-link-to-base ratio. Post-attachment changes in gene and protein expression were assessed using qPCR and immunocytochemistry. We found that while ARPE-19 and iRPE exhibited significant differences in morphology and expression of RPE markers, substrate stiffness did not have a substantial impact on cell growth or maturation for either cell type. These results highlight the differences in expression between immortalized and iPSC-derived RPE cells, and also suggest that stiffnesses in this range (~12-800 kPa) may not result in significant differences in RPE growth and maturation, an important consideration in scaffold design.
Details
- Title: Subtitle
- Influence of Substrate Stiffness on iPSC-Derived Retinal Pigmented Epithelial Cells
- Creators
- Rion J Wendland - University of IowaBudd A Tucker - University of IowaKristan S Worthington - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Stem cells translational medicine, Vol.13(6), pp.582-592
- DOI
- 10.1093/stcltm/szae022
- PMID
- 38560893
- PMCID
- PMC11165161
- NLM abbreviation
- Stem Cells Transl Med
- eISSN
- 2157-6580
- Language
- English
- Electronic publication date
- 04/01/2024
- Date published
- 06/2024
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Chemical and Biochemical Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9984582305402771
Metrics
7 Record Views