Journal article
Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o–: implications for the use of this cell line as an in vitro model for bronchial drug absorption studies
Cell and tissue research, Vol.308(3), pp.391-400
06/2002
DOI: 10.1007/s00441-002-0548-5
PMID: 12107432
Abstract
Air-interfaced culture (AIC) versus liquid-covered culture (LCC) conditions are known to have different effects on the differentiated phenotype of several cell types, including lung epithelial cells. We report the influence of culture conditions such as apical medium volume on the development of intercellular junctions in the human epithelial cell line 16HBE14o–. Immunofluorescence staining of the tight-junctional protein, ZO-1, has revealed its presence in cells grown in both AIC and LCC. However, only LCC-grown cells exhibit protein ZO-1 localized as a zonula-occludens-like regular belt connecting neighboring cells. The presence of typical tight junctions has been confirmed by electron microscopy. Immunostaining for occludin, claudin-1, connexin43, and E-cadherin has demonstrated intercellular junction structures only in the cells in LCC. These morphological findings have been paralleled by higher transepithelial electrical resistance values and similar fluxes of the hydrophilic permeability marker, fluorescein-Na, under LCC compared with AIC conditions. We conclude that the formation of functional 16HBE14o– cell layers requires the presence of an apical fluid volume, in contrast to other culture conditions for airway epithelial cells.
Details
- Title: Subtitle
- Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o–: implications for the use of this cell line as an in vitro model for bronchial drug absorption studies
- Creators
- Carsten Ehrhardt - Saarland UniversityCarsten Kneuer - Saarland UniversityJennifer Fiegel - Johns Hopkins UniversityJustin Hanes - Johns Hopkins UniversityUlrich Schaefer - Saarland UniversityKwang-Jin Kim - Will Rogers Institute Pulmonary Research Center, Division of Pulmonary and Critical Care Medicine, Departments of Medicine, Physiology and Biophysics, Biomedical Engineering, and Molecular Pharmacology and Toxicology, University of Southern California, Los Angeles, CA 90033, USAClaus-Michael Lehr - Saarland University
- Resource Type
- Journal article
- Publication Details
- Cell and tissue research, Vol.308(3), pp.391-400
- Publisher
- Springer-Verlag
- DOI
- 10.1007/s00441-002-0548-5
- PMID
- 12107432
- ISSN
- 0302-766X
- eISSN
- 1432-0878
- Language
- English
- Date published
- 06/2002
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Chemical and Biochemical Engineering
- Record Identifier
- 9984197255102771
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