Journal article
Influence of extracellular matrix macromolecules on normal human keratinocyte phenotype and parathyroid hormone-related protein secretion and expression in vitro
Experimental cell research, Vol.238(1), pp.204-215
01/10/1998
DOI: 10.1006/excr.1997.3830
PMID: 9457073
Abstract
Parathyroid hormone-related protein (PTHrP) is produced by a wide range of neoplastic and normal cells, including keratinocytes where it mag be involved in the regulation of cellular growth and differentiation, There is evidence that the nature of the extracellular matrix (ECM) influences gene expression and cell phenotype, The objective of this study was to investigate the phenotype of normal human keratinocytes grown on different types of ECM (basement membrane components or collagen type I), as well as the expression and secretion of PTHrP. Normal keratinocytes grown on basement membrane extract (Matrigel) actively reorganized the matrix and formed networks of cells with traction of the matrix. This was associated with linear arrays of intracellular microtubules and formation of prominent actin stress fibers and was suppressed by treatment with colchicine or cytochalasin B, confirming the role of the cytoskeleton in this process. In addition, growth on Matrigel was associated with increased PTHrP nuclear translocation, secretion, and mRNA expression compared to growth on collagen where keratinocytes exhibited decreased proliferation and increased differentiation, PTHrP, as a paracrine keratinocyte factor, did not appear to mediate the morphologic changes, since they were not altered by treatment with neutralizing anti-PTHrP antibodies. It was concluded that different types of ECM influenced the morphologic, functional, and proliferative characteristics of keratinocytes, as well as the level of PTHrP expression and secretion in vitro. (C) 1998 Academic Press.
Details
- Title: Subtitle
- Influence of extracellular matrix macromolecules on normal human keratinocyte phenotype and parathyroid hormone-related protein secretion and expression in vitro
- Creators
- EAG BlommeM T Weckmann - The Ohio State UniversityC C Capen - The Ohio State UniversityT J Rosol - The Ohio State University
- Resource Type
- Journal article
- Publication Details
- Experimental cell research, Vol.238(1), pp.204-215
- Publisher
- Elsevier
- DOI
- 10.1006/excr.1997.3830
- PMID
- 9457073
- ISSN
- 0014-4827
- eISSN
- 1090-2422
- Number of pages
- 12
- Grant note
- K04AR001923 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) AR40220; AR01923 / NIAMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS)
- Language
- English
- Date published
- 01/10/1998
- Academic Unit
- Psychiatry; Family and Community Medicine
- Record Identifier
- 9984297341202771
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