Journal article
Influenza A virus infection impacts systemic microbiota dynamics and causes quantitative enteric dysbiosis
Microbiome, Vol.6(1), pp.9-9
01/10/2018
DOI: 10.1186/s40168-017-0386-z
PMCID: PMC5763955
PMID: 29321057
Abstract
Microbiota integrity is essential for a growing number of physiological processes. Consequently, disruption of microbiota homeostasis correlates with a variety of pathological states. Importantly, commensal microbiota provide a shield against invading bacterial pathogens, probably by direct competition. The impact of viral infections on host microbiota composition and dynamics is poorly understood. Influenza A viruses (IAV) are common respiratory pathogens causing acute infections. Here, we show dynamic changes in respiratory and intestinal microbiota over the course of a sublethal IAV infection in a mouse model.
Using a combination of 16S rRNA gene-specific next generation sequencing and qPCR as well as culturing of bacterial organ content, we found body site-specific and transient microbiota responses. In the lower respiratory tract, we observed only minor qualitative changes in microbiota composition. No quantitative impact on bacterial colonization after IAV infection was detectable, despite a robust antimicrobial host response and increased sensitivity to bacterial super infection. In contrast, in the intestine, IAV induced robust depletion of bacterial content, disruption of mucus layer integrity, and higher levels of antimicrobial peptides in Paneth cells. As a functional consequence of IAV-mediated microbiota depletion, we demonstrated that the small intestine is rendered more susceptible to bacterial pathogen invasion, in a Salmonella typhimurium super infection model.
We show for the first time the consequences of IAV infection for lower respiratory tract and intestinal microbiobiota in a qualitative and quantitative fashion. The discrepancy of relative 16S rRNA gene next-generation sequencing (NGS) and normalized 16S rRNA gene-specific qPCR stresses the importance of combining qualitative and quantitative approaches to correctly analyze composition of organ associated microbial communities. The transiently induced dysbiosis underlines the overall stability of microbial communities to effects of acute infection. However, during a short-time window, specific ecological niches might lose their microbiota shield and remain vulnerable to bacterial invasion.
Details
- Title: Subtitle
- Influenza A virus infection impacts systemic microbiota dynamics and causes quantitative enteric dysbiosis
- Creators
- Soner Yildiz - University of GenevaBéryl Mazel-Sanchez - University of GenevaMatheswaran Kandasamy - University of ChicagoBalaji Manicassamy - University of Iowa, Microbiology and ImmunologyMirco Schmolke - Department of Microbiology and Molecular Medicine, University Medical Center (CMU), University of Geneva, Rue Michel-Servet 1, CH-1211, Geneva, Switzerland. mirco.schmolke@unige.ch
- Resource Type
- Journal article
- Publication Details
- Microbiome, Vol.6(1), pp.9-9
- DOI
- 10.1186/s40168-017-0386-z
- PMID
- 29321057
- PMCID
- PMC5763955
- NLM abbreviation
- Microbiome
- ISSN
- 2049-2618
- eISSN
- 2049-2618
- Grant note
- n/a / Foundation Novartis Consumer Health AI12335 / National Institute of Allergy and Infectious Diseases K99 AI095320 / NIAID NIH HHS R00 AI095320 / NIAID NIH HHS R01 AI123359 / NIAID NIH HHS AI095320 / National Institute of Allergy and Infectious Diseases n/a / Fondation Ernst et Lucie Schmidheiny SNF310030_155949 / Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Language
- English
- Date published
- 01/10/2018
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984206855102771
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