Journal article
Inhalation of 2,2’,5,5’-Tetrachlorobiphenyl (PCB52) Causes Changes to the Gut Microbiome Throughout the Gastrointestinal Tract
Journal of hazardous materials, Vol.480, 135999
12/05/2024
DOI: 10.1016/j.jhazmat.2024.135999
PMCID: PMC11608156
PMID: 39369679
Abstract
Polychlorinated biphenyls (PCBs), such as PCB52, are hazardous environmental contaminants present in indoor and outdoor environments. Oral PCB exposure affects the colon microbiome; however, it is unknown if inhalation of PCBs alters the intestinal microbiome. We hypothesize that sub-acute inhalation of PCB52 affects microbial communities depending on the location in the (GI) gastrointestinal tract and the local profiles of PCB52 and its metabolites present in the GI tract following mucociliary clearance and biliary or intestinal excretion. Sprague-Dawley rats were exposed via nose-only inhalation 4hours per day, 7 days per week, for 4 weeks to either filtered air or PCB52. After 28 days, differences in the microbiome and levels of PCB52 and its metabolites were characterized throughout the GI tract. PCB52 inhalation altered taxa abundances and predicted functions altered throughout the gut, with most alterations occurring in the large intestine. PCB52 and metabolite levels varied across the GI tract, resulting in differing PCB × microbiome networks. Thus, the presence of different PCB52 and its metabolites in different parts of the GI tract has varying effects on the composition and predicted function of microbial communities. Future studies need to investigate whether these changes lead to adverse outcomes.
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•Inhalation of PCB 52 in rats alters the taxonomic composition in the cecum and colon.•Most predicted changes at the enzyme levels occur in the jejunum and ileum.•Bacterial alterations correlate with the level of PCB found in the intestinal content throughout the GI tract.
Details
- Title: Subtitle
- Inhalation of 2,2’,5,5’-Tetrachlorobiphenyl (PCB52) Causes Changes to the Gut Microbiome Throughout the Gastrointestinal Tract
- Creators
- Laura E. Dean - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IAHui Wang - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IAAmanda J. Bullert - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IAHui Wang - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IAAndrea Adamcakova-Dodd - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IAAshutosh K. Mangalam - Department of Pathology, University of Iowa, Iowa City, IAPeter S. Thorne - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IAJames A. Ankrum - Department of Biomedical Engineering, University of Iowa, Iowa City, IAAloysius J. Klingelhutz - Department of Microbiology and Immunology, University of Iowa, Iowa City, IAHans-Joachim Lehmler - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA
- Resource Type
- Journal article
- Publication Details
- Journal of hazardous materials, Vol.480, 135999
- DOI
- 10.1016/j.jhazmat.2024.135999
- PMID
- 39369679
- PMCID
- PMC11608156
- NLM abbreviation
- J Hazard Mater
- ISSN
- 0304-3894
- eISSN
- 1873-3336
- Publisher
- Elsevier B.V
- Grant note
- National Institute of Environmental Health Sciences: R01ES014901, R01ES031098, R01ES034691, P30ES005605, P42ES013661
The work was supported by the National Institute of Environmental Health Sciences [grant numbers R01ES014901, R01ES031098, R01ES034691, P30ES005605, and P42ES013661] . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Environmental Health Sciences.
- Language
- English
- Electronic publication date
- 10/01/2024
- Date published
- 12/05/2024
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Microbiology and Immunology; Occupational and Environmental Health; Pathology; Iowa Neuroscience Institute; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Iowa Superfund Research Program
- Record Identifier
- 9984721144102771
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