Journal article
Inhibition of Cell Growth by Overexpression of Manganese Superoxide Dismutase (MnSOD) in Human Pancreatic Carcinoma
Free radical research, Vol.38(11), pp.1223-1233
2004
DOI: 10.1080/10715760400017376
PMID: 15621700
Abstract
Manganese superoxide dismutase (MnSOD) levels have been found to be low in human pancreatic cancer [Pancreas26, (2003), 23] and human pancreatic cancer cell lines [Cancer Res.63, (2003), 1297] when compared to normal human pancreas. We hypothesized that stable overexpression of pancreatic cancer cells with MnSOD cDNA would alter the malignant phenotype. MIA PaCa-2 cells were stably transfected with a pcDNA3 plasmid containing sense human MnSOD cDNA or containing no MnSOD insert by using the lipofectAMINE method. G418-resistant colonies were isolated, grown and maintained. Overexpression of MnSOD was confirmed in two selected clones with a 2-4-fold increase in MnSOD immunoreactive protein. Compared with the parental and neo control cells, the MnSOD-overexpressing clones had decreased growth rates, growth in soft agar and plating efficiency in vitro, while in vivo, the MnSOD-overexpressing clones had slower growth in nude mice. These results suggest that MnSOD may be a tumor suppressor gene in human pancreatic cancer.
Details
- Title: Subtitle
- Inhibition of Cell Growth by Overexpression of Manganese Superoxide Dismutase (MnSOD) in Human Pancreatic Carcinoma
- Creators
- Matthew Ough - 1University of Iowa College of Medicine Iowa City IA USAAnne Lewis - 1University of Iowa College of Medicine Iowa City IA USAYuping Zhang - 2Radiation Oncology Iowa City IA USAMarilyn M Hinkhouse - 3Veterans Affairs Medical Center Iowa City IA USAJustine M Ritchie - 4The Holden Comprehensive Cancer Center Iowa City IA USALarry W OberleyJoseph J Cullen
- Resource Type
- Journal article
- Publication Details
- Free radical research, Vol.38(11), pp.1223-1233
- Publisher
- Informa UK Ltd
- DOI
- 10.1080/10715760400017376
- PMID
- 15621700
- ISSN
- 1071-5762
- eISSN
- 1029-2470
- Language
- English
- Date published
- 2004
- Academic Unit
- Surgery; Radiation Oncology; Obstetrics and Gynecology
- Record Identifier
- 9984051883902771
Metrics
33 Record Views