Inhibition of HIV Type 1 Replication by Human T Lymphotropic Virus Types 1 and 2 Tax Proteins in Vitro

Christy S Barrios; Laura Castillo; Chou-Zen Giam; Li Wu; Mark A Beilke

doi:10.1089/aid.2013.0027

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Inhibition of HIV Type 1 Replication by Human T Lymphotropic Virus Types 1 and 2 Tax Proteins in Vitro

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Inhibition of HIV Type 1 Replication by Human T Lymphotropic Virus Types 1 and 2 Tax Proteins in Vitro

Christy S Barrios, Laura Castillo, Chou-Zen Giam, Li Wu and Mark A Beilke

AIDS research and human retroviruses, Vol.29(7), pp.161-1067

07/01/2013

DOI: 10.1089/aid.2013.0027

PMCID: PMC3685691

PMID: 23464580

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Abstract

Patients with HIV-1 and human T-lymphotropic virus type 2 (HTLV-2) coinfections often exhibit a clinical course similar to that seen in HIV-1-infected individuals who are long-term nonprogressors. These findings have been attributed in part to the ability of HTLV-2 to activate production of antiviral chemokines and to downregulate the CCR5 coreceptor on lymphocytes. To further investigate these observations, we tested the ability of recombinant Tax1 and Tax2 proteins to suppress HIV-1 viral replication in vitro . R5-tropic HIV-1 (NLAD8)-infected peripheral blood mononuclear cells (PBMCs) were treated daily with recombinant Tax1 and Tax2 proteins (dosage range 1–100 pM). Culture supernatants were collected at intervals from days 1 to 22 postinfection and assayed for levels of HIV-1 p24 antigen by ELISA. Treatment of PBMCs with Tax2 protein resulted in a significant reduction in HIV-1 p24 antigen levels ( p <0.05) at days 10, 14, and 18 postinfection compared to HIV-1-infected or mock-treated PBMCs. This was preceded by the detection of increased levels of CC-chemokines MIP-1α/CCL3, MIP-1β/CCL4, and RANTES/CCL5 on days 1–7 of infection. Similar, but less robust inhibition was observed in Tax1-treated PBMCs. These results support the contention that Tax1 and Tax2 play a role in generating antiviral responses against HIV-1 in vivo and in vitro .

Pathogenesis

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Title: Subtitle: Inhibition of HIV Type 1 Replication by Human T Lymphotropic Virus Types 1 and 2 Tax Proteins in Vitro
Creators: Christy S Barrios - 2Clement J. Zablocki Veterans Affairs Medical Center, Milwaukee, Wisconsin
Laura Castillo - 2Clement J. Zablocki Veterans Affairs Medical Center, Milwaukee, Wisconsin
Chou-Zen Giam - 3Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland
Li Wu - 4Center for Retrovirus Research, Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio
Mark A Beilke - 2Clement J. Zablocki Veterans Affairs Medical Center, Milwaukee, Wisconsin
Resource Type: Journal article
Publication Details: AIDS research and human retroviruses, Vol.29(7), pp.161-1067
DOI: 10.1089/aid.2013.0027
PMID: 23464580
PMCID: PMC3685691
NLM abbreviation: AIDS Res Hum Retroviruses
ISSN: 0889-2229
eISSN: 1931-8405
Publisher: Mary Ann Liebert, Inc
Language: English
Date published: 07/01/2013
Academic Unit: Microbiology and Immunology
Record Identifier: 9984002374702771

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