Journal article
Inhibition of RNA splicing at the Rous sarcoma virus src 3' splice site is mediated by an interaction between a negative cis element and a chicken embryo fibroblast nuclear factor
Journal of virology, Vol.69(8), pp.5068-5076
08/1995
DOI: 10.1128/jvi.69.8.5068-5076.1995
PMCID: PMC189324
PMID: 7609076
Abstract
In permissive Rous sarcoma virus-infected chicken embryo fibroblasts (CEF), approximately equimolar amounts of env and src mRNAs are present. In nonpermissive mammalian cells, the src mRNA level is elevated and env mRNA level is reduced. A cis element in the region between the env gene and the src 3' splice site, which we have termed the suppressor of src splicing (SSS), acts specifically in CEF but not in human cells to reduce src mRNA levels. The splicing inhibition in CEF is not caused by a base-paired structure which is predicted to form between the SSS and the src 3' splice site. To further investigate the mechanism of the inhibition, we have used human HeLa cell nuclear extracts to compare in vitro the rates of splicing of RNA substrates containing the Rous sarcoma virus major 5' splice site and either the env or src 3' splice sites. We show that the src 3' splice site is used approximately fivefold more efficiently than the env 3' splice site. The efficiency of in vitro splicing at the src 3' splice site is specifically reduced by addition of CEF nuclear extract. The inhibition is dependent on the presence of the SSS element and can be abrogated by addition of competitor RNA. We propose that the SSS region represents a binding site for a negative-acting CEF splicing factor(s).
Details
- Title: Subtitle
- Inhibition of RNA splicing at the Rous sarcoma virus src 3' splice site is mediated by an interaction between a negative cis element and a chicken embryo fibroblast nuclear factor
- Creators
- B A Amendt - University of IowaS B Simpson - University of IowaC M Stoltzfus - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.69(8), pp.5068-5076
- DOI
- 10.1128/jvi.69.8.5068-5076.1995
- PMID
- 7609076
- PMCID
- PMC189324
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 08/1995
- Academic Unit
- Orthodontics; Microbiology and Immunology; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984284358802771
Metrics
15 Record Views