Journal article
Inhibition of cell growth and sensitization to oxidative damage by overexpression of manganese superoxide dismutase in rat glioma cells
Cell growth & differentiation, Vol.7(9), pp.1175-1186
1996
PMID: 8877099
Abstract
The effects of overexpression of human manganese superoxide dismutase (MnSOD) on cell proliferation and response to oxidative stress in rat glioma cells were studied. MnSOD-overexpressing cells had a 2- to 14-fold increase in MnSOD activity, but did not have consistent changes in the activities of CuZnSOD, catalase, or glutathione peroxidase. Cells with more than a 5-fold increase in MnSOD activity became more sensitive to radiation, 1,3-bis(2-chloroethyl)-1-nitrosourea, and buthionine sulfoximine and had a lower growth rate than parental and vector control cells. The sensitivity to 1,3-bis(2-chloroethyl)-1-nitrosourea was partially reduced by pyruvate, a H2O2 scavenger. Our results suggest that overexpression of MnSOD can cause an imbalance of antioxidant enzymes, which we hypothesize results in an elevation of intracellular H2O2. Overexpression of MnSOD can either inhibit cell proliferation or increase cell death by oxidative agents, depending on the levels of peroxide-removing enzymes.
Details
- Title: Subtitle
- Inhibition of cell growth and sensitization to oxidative damage by overexpression of manganese superoxide dismutase in rat glioma cells
- Creators
- W ZHONG - Radiation Research Laboratory, The University of Iowa, Iowa City, Iowa 52242, United StatesL. W OBERLEY - Radiation Research Laboratory, The University of Iowa, Iowa City, Iowa 52242, United StatesT. D OBERLEY - Pathology Service, William S. Middleton Veterans Memorial Hospital and Department of Pathology, University of Wisconsin, Madison, Wisconsin 53705, United StatesT YAN - Radiation Research Laboratory, The University of Iowa, Iowa City, Iowa 52242, United StatesF. E DOMANN - Radiation Research Laboratory, The University of Iowa, Iowa City, Iowa 52242, United StatesD. K. S CLAIR - Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536, United States
- Resource Type
- Journal article
- Publication Details
- Cell growth & differentiation, Vol.7(9), pp.1175-1186
- Publisher
- American Association for Cancer Research; Philadelphia, PA
- PMID
- 8877099
- ISSN
- 1044-9523
- eISSN
- 2377-0732
- Language
- English
- Date published
- 1996
- Academic Unit
- Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984046911302771
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