Inhibition of cytochromes P450 and the hydroxylation of 4-monochlorobiphenyl in whole poplar

Guangshu Zhai; Hans-Joachim Lehmler; Jerald L Schnoor

doi:10.1021/es304298m

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Inhibition of cytochromes P450 and the hydroxylation of 4-monochlorobiphenyl in whole poplar

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Inhibition of cytochromes P450 and the hydroxylation of 4-monochlorobiphenyl in whole poplar

Guangshu Zhai, Hans-Joachim Lehmler and Jerald L Schnoor

Environmental science & technology, Vol.47(13), pp.6829-6835

07/02/2013

DOI: 10.1021/es304298m

PMCID: PMC3652898

PMID: 23320482

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Abstract

Cytochromes P450 (CYPs) are potential enzymes responsible for hydroxylation of many xenobiotics and endogenous chemicals in living organisms. It has been found that 4-monochlorobiphenyl (PCB3), mainly an airborne pollutant, can be metabolized to hydroxylated transformation products (OH-PCB3s) in whole poplars. However, the enzymes involved in the hydroxylation of PCB3 in whole poplars have not been identified. Therefore, two CYP suicide inhibitors, 1-aminobenzotriazole (ABT) and 17-octadecynoic acid (ODYA), were selected to probe the hydroxylation reaction of PCB3 in whole poplars in this work. Poplars (Populus deltoides × nigra, DN34) were exposed to PCB3 with or without inhibitor for 11 days. Results showed both ABT and ODYA can decrease the concentrations and yields of five OH-PCB3s in different poplar parts via the inhibition of CYPs. Furthermore, both ABT and ODYA demonstrated a dose-dependent relationship to the formation of OH-PCB3s in whole poplars. The higher the inhibitor concentrations, the lower the total yields of OH-PCB3s. For ABT spiked-additions, the total mass yield of five OH-PCB3s was inhibited by a factor of 1.6 times at an ABT concentration of 2.5 mg L(-1), 4.0 times at 12.5 mg L(-1), and 7.0 times at 25 mg L(-1). For the inhibitor ODYA, the total mass of five OH-PCB3s was reduced by 2.1 times compared to the control at an ODYA concentration of 2.5 mg L(-1). All results pointed to the conclusion that CYP enzymes were the agents which metabolized PCB3 to OH-PCB3s in whole poplars because suicide CYP inhibitors ABT and ODYA both led to sharp decreases of OH-PCB3s formation in whole poplars. A dose-response curve for each of the suicide inhibitors was developed.

Triazoles - pharmacology

Cytochrome P-450 Enzyme Inhibitors

Populus - metabolism

Enzyme Inhibitors - pharmacology

Cytochrome P-450 Enzyme System - metabolism

Fatty Acids, Unsaturated - pharmacology

Environmental Pollutants - metabolism

Populus - drug effects

Biphenyl Compounds - metabolism

Details

Title: Subtitle: Inhibition of cytochromes P450 and the hydroxylation of 4-monochlorobiphenyl in whole poplar
Creators: Guangshu Zhai - Department of Civil and Environmental Engineering and IIHR Hydroscience and Engineering, The University of Iowa, Iowa City, Iowa 52242, United States. zhai-guangshu@uiowa.edu
Hans-Joachim Lehmler
Jerald L Schnoor
Resource Type: Journal article
Publication Details: Environmental science & technology, Vol.47(13), pp.6829-6835
DOI: 10.1021/es304298m
PMID: 23320482
PMCID: PMC3652898
NLM abbreviation: Environ Sci Technol
ISSN: 1520-5851
eISSN: 1520-5851
Publisher: United States
Grant note: P42 ES013661 / NIEHS NIH HHS P42ES013661 / NIEHS NIH HHS P30 ES005605 / NIEHS NIH HHS
Language: English
Date published: 07/02/2013
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; Iowa Neuroscience Institute; Iowa Superfund Research Program
Record Identifier: 9983991969702771

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